CNTF Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83277

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CNTF Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FHQAIHTLLLQVAAFAYQIEELMILLEYKIPRNEADGMPINVGDGGLFEKKLWGLKVLQELSQWTVRSIHDLRFISSHQTGIPARGSHYIANNK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (84%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit CNTF Antibody - BSA Free (NBP1-83277) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CNTF Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CNTF Antibody [NBP1-83277]

Immunocytochemistry/ Immunofluorescence: CNTF Antibody [NBP1-83277]

Immunocytochemistry/Immunofluorescence: CNTF Antibody [NBP1-83277] - Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CNTF Antibody [NBP1-83277]

Immunohistochemistry-Paraffin: CNTF Antibody [NBP1-83277]

Immunohistochemistry-Paraffin: CNTF Antibody [NBP1-83277] - Staining of human Skeletal muscle shows very weak cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: CNTF Antibody [NBP1-83277]

Immunohistochemistry-Paraffin: CNTF Antibody [NBP1-83277]

Immunohistochemistry-Paraffin: CNTF Antibody [NBP1-83277] - Staining of human Cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: CNTF Antibody [NBP1-83277]

Immunohistochemistry-Paraffin: CNTF Antibody [NBP1-83277]

Immunohistochemistry-Paraffin: CNTF Antibody [NBP1-83277] - Staining of human Cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: CNTF Antibody [NBP1-83277]

Immunohistochemistry-Paraffin: CNTF Antibody [NBP1-83277]

Immunohistochemistry-Paraffin: CNTF Antibody [NBP1-83277] - Staining of human Skin shows very weak cytoplasmic positivity in squamous epithelial cells.
CNTF Antibody - BSA Free Western Blot: CNTF Antibody - BSA Free [NBP1-83277]

Western Blot: CNTF Antibody - BSA Free [NBP1-83277]

Analysis in human cell line U-87 MG.

Applications for CNTF Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CNTF

CNTF is a survival promoting factor for different types of neurons in vitro and in vivo. The essential structural features for the biological function of human CNTF were investigated by Thier, M. et al. They showed that deletion of 14 N-terminal and 18 C-terminal amino acids significantly increased bioactivity compared to wild-type CNTF. FUNCTION: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Nervous system. PHARMACEUTICAL: CNTF is being tested under the name Axokine by Regeneron Pharmaceuticals for treatment of human motor neuron diseases, such as amyotrophic lateral sclerosis (ALS). As it induces substantial weight loss, preferentially of fat as opposed to lean body mass, it is being used for obesity treatment. SIMILARITY: Belongs to the CNTF family.

Long Name

Ciliary Neurotrophic Factor

Alternate Names

ciliary neurotrophic factor, HCNTF

Gene Symbol

CNTF

Additional CNTF Products

Product Documents for CNTF Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CNTF Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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