COASY Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32649

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all COASY Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human COASY. The exact sequence is proprietary.

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for COASY Antibody - BSA Free

Western Blot: COASY Antibody [NBP1-32649]

Western Blot: COASY Antibody [NBP1-32649]

Western Blot: COASY Antibody [NBP1-32649] - Sample (50 ug of whole cell lysate) A: mouse liver 7. 5% SDS PAGE, antibody diluted at 1:3000.
Immunocytochemistry/ Immunofluorescence: COASY Antibody [NBP1-32649]

Immunocytochemistry/ Immunofluorescence: COASY Antibody [NBP1-32649]

Immunocytochemistry/Immunofluorescence: COASY Antibody [NBP1-32649] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: COASY Antibody [NBP1-32649]

Immunohistochemistry-Paraffin: COASY Antibody [NBP1-32649]

Immunohistochemistry-Paraffin: COASY Antibody [NBP1-32649] - COASY antibody detects COASY protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. COASY antibody diluted at 1:500.
Western Blot: COASY Antibody [NBP1-32649]

Western Blot: COASY Antibody [NBP1-32649]

Western Blot: COASY Antibody [NBP1-32649] - (30 ug whole cell lysate) A: HeLa S3 7.5% SDS PAGE diluted at 1:1000
Immunohistochemistry-Paraffin: COASY Antibody [NBP1-32649]

Immunohistochemistry-Paraffin: COASY Antibody [NBP1-32649]

Immunohistochemistry-Paraffin: COASY Antibody [NBP1-32649] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: COASY Antibody [NBP1-32649]

Immunohistochemistry-Paraffin: COASY Antibody [NBP1-32649]

Immunohistochemistry-Paraffin: COASY Antibody [NBP1-32649] - COASY Antibody detects COASY protein at cytoplasm in mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. COASY antibody diluted at 1:500.

Applications for COASY Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: COASY

Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM]

Alternate Names

bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase, CoA synthaseUKR1, CoASY, Coenzyme A synthase, DPCK, FLJ35179, NBPbifunctional coenzyme A synthase, nucleotide binding protein, phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase, POV-2, PPAT

Gene Symbol

COASY

Additional COASY Products

Product Documents for COASY Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for COASY Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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