Cofilin 2 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-98910

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Cofilin 2 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Cofilin 2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Cofilin 2 Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: Cofilin 2 Antibody [NBP2-98910]

Immunohistochemistry-Paraffin: Cofilin 2 Antibody [NBP2-98910]

Immunohistochemistry-Paraffin: Cofilin 2 Antibody [NBP2-98910] - Immunochemical staining Cofilin 2 in mouse skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Immunohistochemistry-Paraffin: Cofilin 2 Antibody [NBP2-98910]

Immunohistochemistry-Paraffin: Cofilin 2 Antibody [NBP2-98910]

Immunohistochemistry-Paraffin: Cofilin 2 Antibody [NBP2-98910] - Immunochemical staining Cofilin 2 in human skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Cofilin 2 Antibody

Immunoprecipitation: Cofilin 2 Antibody [NBP2-98910] -

Immunoprecipitation: Cofilin 2 Antibody [NBP2-98910] -Lane A:0.5 mg Raji Whole Cell LysateLane B:0.5 mg Hela Whole Cell Lysate4 µL anti-CFL2 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:Anti-CFL2 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 18 kDaObserved band size: 18 kDa
Cofilin 2 Antibody

Western Blot: Cofilin 2 Antibody [NBP2-98910] -

Western Blot: Cofilin 2 Antibody [NBP2-98910] -Lane A: Raji Whole Cell LysateLane B: Hela Whole Cell LysateLane C: NCI-H1299 Whole Cell LysateLysates/proteins at 30 μg per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:18 kDaObserved band size:18 kDa(We are unsure as to the identity of these extra bands.)
Cofilin 2 Antibody - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Cofilin 2 Antibody [NBP2-98910] -

Immunocytochemistry/Immunofluorescence: Cofilin 2 Antibody [NBP2-98910] - Immunofluorescence staining of Cofilin 2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98910 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.

Applications for Cofilin 2 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunohistochemistry

1:500-1:2000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cofilin 2

Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. (Gillett et al., 1996 [PubMed 8800436]). Cofilin-2 is a member of the AC group of proteins that also includes cofilin-1 (CFL1) and destrin (DSTN; MIM 609114), all of which regulate actin-filament dynamics (Bamburg et al., 1999 [PubMed 10461190]; Maciver and Hussey, 2002). The CFL2 gene encodes a skeletal muscle-specific isoform (Vartiainen et al., 2002 [PubMed 11809832]) localized to the thin filaments, where it exerts its effect on actin, in part through interactions with tropomyosins (Ono and Ono, 2002 [PubMed 11901171]).[supplied by OMIM]

Alternate Names

cofilin 2 (muscle), Cofilin, muscle isoform, cofilin-2, NEM7

Gene Symbol

CFL2

Additional Cofilin 2 Products

Product Documents for Cofilin 2 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Cofilin 2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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