Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1C1

Format

BSA Free
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Product Specifications

Immunogen

Recombinant human Cofilin (1-166aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Cofilin Antibody (1C1) - BSA Free

Western Blot: Cofilin Antibody (1C1)BSA Free [NBP1-21044]

Western Blot: Cofilin Antibody (1C1)BSA Free [NBP1-21044]

Western Blot: Cofilin Antibody (1C1) [NBP1-21044] - The lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cofilin 1(1:1000) antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: K562 cell lysateLane 3.: MCF7 cell lysateLane 4.: LnCap cell lysateLane 5.: PC3 cell lysate
Immunocytochemistry/ Immunofluorescence: Cofilin Antibody (1C1) - BSA Free [NBP1-21044]

Immunocytochemistry/ Immunofluorescence: Cofilin Antibody (1C1) - BSA Free [NBP1-21044]

Immunocytochemistry/Immunofluorescence: Cofilin Antibody (1C1) [NBP1-21044] - Analysis of Cofilin1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Cofilin1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Applications for Cofilin Antibody (1C1) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000-1:2000

Reviewed Applications

Read 1 review rated 1 using NBP1-21044 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CFL1

Cofilin is a small phosphoinositide sensitive, actin binding protein capable of depolymerizing actin filaments in vitro. Under certain conditions, it fragments the filaments and accelerates actin subunit dissociation from their 'pointed' (minus) ends. Cofilin binds stoichiometrically to monomeric G-actin and to actin protomers in filaments in an apparent pH-dependent, Ca2+ independent manner. Cofilin intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist. Cofilin is ubiquitous in tissues of eukaryotes and is especially abundant in neuronal tissues. It is essential for viability and is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, cytokinesis, and pathogen intracellular motility.

Long Name

cofilin 1

Alternate Names

CFL, cofilin, cofilin-1, HEL-S-15

Entrez Gene IDs

1072 (Human)

Gene Symbol

CFL1

UniProt

NP_005498 (Human)

Additional CFL1 Products

Product Documents for Cofilin Antibody (1C1) - BSA Free

Certificate of Analysis

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Product Specific Notices for Cofilin Antibody (1C1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Cofilin Antibody (1C1) - BSA Free (1)

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  • Cofilin Antibody (1C1)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human trabecular meshwork cells
    Species: Human
    Verified Customer | Posted 12/18/2018
    Unfortunately, this antibody did nor work for my experiment while another company's ab did.
    Cofilin Antibody (1C1) - BSA Free NBP1-21044

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