Cofilin Antibody (GT217) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-13520
![Western Blot: Cofilin Antibody (GT217) [NBP3-13520]](https://resources.rndsystems.com/images/products/Cofilin-Antibody-GT217-Western-Blot-NBP3-13520-img0006.jpg "Western Blot: Cofilin Antibody (GT217) [NBP3-13520]")
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Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (99%), Porcine (99%), Rhesus Macaque (99%), Sheep (99%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # GT217
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Cofilin. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Description
Centrifuge briefly prior to opening.
Scientific Data Images for Cofilin Antibody (GT217) - Azide and BSA Free
Western Blot: Cofilin Antibody (GT217) [NBP3-13520]
Western Blot: Cofilin Antibody (GT217) [NBP3-13520] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin antibody [GT217] (NBP3-13520) diluted at 1:1000.![Immunohistochemistry-Paraffin: Cofilin Antibody (GT217) [NBP3-13520]](https://resources.rndsystems.com/images/products/Cofilin-Antibody-GT217-Immunohistochemistry-Paraffin-NBP3-13520-img0003.jpg "Immunohistochemistry-Paraffin: Cofilin Antibody (GT217) [NBP3-13520]")
Immunohistochemistry-Paraffin: Cofilin Antibody (GT217) [NBP3-13520]
Immunohistochemistry-Paraffin: Cofilin Antibody (GT217) [NBP3-13520] - Cofilin antibody [GT217] detects Cofilin protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Cofilin antibody [GT217] (NBP3-13520) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min![Western Blot: Cofilin Antibody (GT217) [NBP3-13520]](https://resources.rndsystems.com/images/products/Cofilin-Antibody-GT217-Western-Blot-NBP3-13520-img0004.jpg "Western Blot: Cofilin Antibody (GT217) [NBP3-13520]")
Western Blot: Cofilin Antibody (GT217) [NBP3-13520]
Western Blot: Cofilin Antibody (GT217) [NBP3-13520] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin antibody [GT217] (NBP3-13520) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.![Western Blot: Cofilin Antibody (GT217) [NBP3-13520]](https://resources.rndsystems.com/images/products/Cofilin-Antibody-GT217-Western-Blot-NBP3-13520-img0005.jpg "Western Blot: Cofilin Antibody (GT217) [NBP3-13520]")
Western Blot: Cofilin Antibody (GT217) [NBP3-13520]
Western Blot: Cofilin Antibody (GT217) [NBP3-13520] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin antibody [GT217] (NBP3-13520) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.![Immunoprecipitation: Cofilin Antibody (GT217) [NBP3-13520]](https://resources.rndsystems.com/images/products/Cofilin-Antibody-GT217-Immunoprecipitation-NBP3-13520-img0002.jpg "Immunoprecipitation: Cofilin Antibody (GT217) [NBP3-13520]")
Immunoprecipitation: Cofilin Antibody (GT217) [NBP3-13520]
Immunoprecipitation: Cofilin Antibody (GT217) [NBP3-13520] - Immunoprecipitation of Cofilin protein from 293T whole cell extracts using 5 ug of Cofilin antibody [GT217] (NBP3-13520). Western blot analysis was performed using Cofilin antibody [GT217] (NBP3-13520) diluted at 1:500. EasyBlot anti-Mouse IgG was used as a secondary reagent.
Western Blot: Cofilin Antibody (GT217) [NBP3-13520] -
Western Blot: Cofilin Antibody (GT217) [NBP3-13520] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin antibody [GT217] (NBP3-13520) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Cofilin Antibody (GT217) [NBP3-13520] -
Immunocytochemistry/ Immunofluorescence: Cofilin Antibody (GT217) [NBP3-13520] - Cofilin antibody [GT217] detects Cofilin protein at cell membrane by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cofilin stained by Cofilin antibody [GT217] (NBP3-13520) diluted at 1:500.
Blue: Fluoroshield with DAPI.
Applications for Cofilin Antibody (GT217) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 20% Glycerol
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CFL1
Long Name
cofilin 1
Alternate Names
CFL, cofilin, cofilin-1, HEL-S-15
Gene Symbol
CFL1
Additional CFL1 Products
Product Documents for Cofilin Antibody (GT217) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Cofilin Antibody (GT217) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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