Cofilin Antibody (PSH01-44)

Western Blot: Cofilin Antibody (PSH01-44) [NBP3-32207]

Western Blot: Cofilin Antibody (PSH01-44) [NBP3-32207] - Western blot analysis of Cofilin on different lysates with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/1,000 dilution.

Lane 1: Hela cell lysate (30 ug/Lane)
Lane 2: HEK-293 cell lysate (30 ug/Lane)
Lane 3: MCF7 cell lysate (30 ug/Lane)
Lane 4: MDA-MB-468 cell lysate (30 ug/Lane)
Lane 5: SH-SY5Y cell lysate (30 ug/Lane)
Lane 6: HUVEC cell lysate (30 ug/Lane)
Lane 7: Jurkat cell lysate (30 ug/Lane)
Lane 8: COS-1 cell lysate (30 ug/Lane)
Lane 9: VERO cell lysate (30 ug/Lane)
Lane 10: NIH/3T3 cell lysate (30 ug/Lane)
Lane 11: PC-12 cell lysate (30 ug/Lane)
Lane 12: Neuro-2a cell lysate (30 ug/Lane)
Lane 13: K-562 cell lysate (30 ug/Lane)
Lane 14: A431 cell lysate (30 ug/Lane)

Predicted band size: 19 kDa
Observed band size: 19 kDa

Exposure time: 2 minutes 37 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32207) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Cofilin Antibody (PSH01-44) [NBP3-32207]

Immunohistochemistry: Cofilin Antibody (PSH01-44) [NBP3-32207] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32207) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: Cofilin Antibody (PSH01-44) [NBP3-32207]

Immunocytochemistry/ Immunofluorescence: Cofilin Antibody (PSH01-44) [NBP3-32207] - Immunocytochemistry analysis of HeLa cells labeling Cofilin with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.