Cofilin Antibody

Name: Cofilin Antibody
Brand: Novus Biologicals
SKU: NBP3-23230
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-23230

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Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat, Chicken

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all CFL1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide (coupled to KLH) corresponding to amino acid residues at the N-terminus of human Cofilin. This sequence has 100% homology with similar regions of rat and mouse Cofilin, and has 3 amino acid differences from human Cofilin 2. [UniProt# P23528]

Specificity

The antibody detects a 19 kDa protein corresponding to the molecular mass of Cofilin on SDS-PAGE immunoblots of Jurkat cells.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for Cofilin Antibody

Western Blot: Cofilin Antibody [NBP3-23230] -

Western Blot: Cofilin Antibody [NBP3-23230] - Western blot of Cofilin in jurkat cells. The blots were untreated (lanes 1 & 4) or treated (lanes 2, 3, 5 & 6) with lambda phosphatase. In lanes 3 & 6, the phosphatase was inhibited with phospho-Cofilin (Ser-3) peptide. The blots were probed with anti-Cofilin (Ser-3) phospho-specific (lanes 1-3) or anti-Cofilin (N-terminus) (lanes 4-6).

Applications for Cofilin Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: CFL1

Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM]

Long Name

cofilin 1

Alternate Names

CFL, cofilin, cofilin-1, HEL-S-15

Gene Symbol

CFL1

Additional CFL1 Products

Product Documents for Cofilin Antibody

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Certificate of Analysis

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Product Specific Notices for Cofilin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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