COG7 Antibody

Novus Biologicals | Catalog # NBP1-05032

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Peptide with sequence C-KTRPEDYRQVSK corresponding to internal region according to NP_705831.1.

Epitope

internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for COG7 Antibody

Western Blot: COG7 Antibody [NBP1-05032]

Western Blot: COG7 Antibody [NBP1-05032]

Western Blot: COG7 Antibody [NBP1-05032] - (0.03ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: COG7 Antibody [NBP1-05032]

Immunohistochemistry-Paraffin: COG7 Antibody [NBP1-05032]

Immunohistochemistry-Paraffin: COG7 Antibody [NBP1-05032] - (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for COG7 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.03-0.1 ug/ml
Application Notes
Western blot: Approx. 85kDa band observed in lysates cell lines HeLa, Jurkat and MOLT4 (calculated MW of 86.3kDa according to NP_705831.1). Primary incubation was 1 hour.
IHC: Paraffin embedded Human Testis.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: COG7

Multiprotein complexes are key determinants of Golgi apparatus structure and its capacity for intracellular transport and glycoprotein modification. Several complexes have been identified, including the Golgi transport complex (GTC), the LDLC complex, which is involved in glycosylation reactions, and the SEC34 complex, which is involved in vesicular transport. These 3 complexes are identical and have been termed the conserved oligomeric Golgi (COG) complex, which includes COG7 (Ungar et al., 2002 [PubMed 11980916]).[supplied by OMIM]

Alternate Names

CDG2E, component of oligomeric golgi complex 7COG complex subunit 7, conserved oligomeric Golgi complex subunit 7

Entrez Gene IDs

91949 (Human)

Gene Symbol

COG7

Additional COG7 Products

Product Documents for COG7 Antibody

Certificate of Analysis

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Product Specific Notices for COG7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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