Coilin Antibody (HL2384)

Name: Coilin Antibody (HL2384)
Brand: Novus Biologicals
SKU: NBP3-48679
Price: 529 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-48679

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2384

View all Coilin Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human Coilin.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Coilin Antibody (HL2384)

Western Blot: Coilin Antibody (HL2384) [NBP3-48679] -

HeLa whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Coilin antibody [HL2384] (NBP3-48679) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/ Immunofluorescence: Coilin Antibody (HL2384) [NBP3-48679] -

Coilin antibody [HL2384] detects Coilin protein at Cajal bodies by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: Coilin stained by Coilin antibody [HL2384] (NBP3-48679) diluted at 1:500.
Blue: Fluoroshield with DAPI.

Applications for Coilin Antibody (HL2384)

Application

Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Coilin

Coilin is encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and chromosome 14.

Alternate Names

CLN80p80-coilin, coilin, coilin p80, p80

Gene Symbol

COIL

Additional Coilin Products

Product Documents for Coilin Antibody (HL2384)

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Certificate of Analysis

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Product Specific Notices for Coilin Antibody (HL2384)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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