Coilin Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15939

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Rhesus Macaque (95%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Coilin Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Coilin. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Coilin Antibody - BSA Free

Western Blot: Coilin Antibody [NBP2-15939]

Western Blot: Coilin Antibody [NBP2-15939]

Western Blot: Coilin Antibody [NBP2-15939] - A. 30 ug 293T whole cell extract B. 30 ug 293T nuclear extract 7.5 % SDS-PAGECoilin antibody dilution: 1:1000
Immunocytochemistry/ Immunofluorescence: Coilin Antibody [NBP2-15939]

Immunocytochemistry/ Immunofluorescence: Coilin Antibody [NBP2-15939]

Immunocytochemistry/Immunofluorescence: Coilin Antibody [NBP2-15939] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using Coilin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:500.
Immunohistochemistry-Paraffin: Coilin Antibody [NBP2-15939]

Immunohistochemistry-Paraffin: Coilin Antibody [NBP2-15939]

Immunohistochemistry-Paraffin: Coilin Antibody [NBP2-15939] - Paraffin-embedded human cervical cancer. Coilin antibody diluted at 1:500
Western Blot: Coilin Antibody [NBP2-15939]

Western Blot: Coilin Antibody [NBP2-15939]

Western Blot: Coilin Antibody [NBP2-15939] - A. 30 ug HepG2 whole cell extract B. 30 ug HepG2 nuclear extract 7.5 % SDS-PAGE Coilin antibody dilution: 1:1000
Immunoprecipitation: Coilin Antibody [NBP2-15939]

Immunoprecipitation: Coilin Antibody [NBP2-15939]

Immunoprecipitation: Coilin Antibody [NBP2-15939] - Immunoprecipitation of Coilin protein from 293T whole cell extracts using 5 ug of Coilin antibody. Western blot analysis was performed using Coilin antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Coilin Antibody

Western Blot: Coilin Antibody [NBP2-15939] -

Western Blot: Coilin Antibody [NBP2-15939] - HeLa whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Coilin antibody diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for Coilin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Coilin

The protein encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and chromosome 14. [provided by RefSeq]

Alternate Names

CLN80p80-coilin, coilin, coilin p80, p80

Gene Symbol

COIL

UniProt

P38432 (Human)

Additional Coilin Products

Product Documents for Coilin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Coilin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Coilin Antibody - BSA Free

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Protocols

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FAQs

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