COLEC10 Antibody (4C1) - Azide and BSA Free

Novus Biologicals | Catalog # H00010584-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4C1

Format

Azide and BSA Free
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Product Specifications

Immunogen

COLEC10 (NP_006429.1, 121 a.a. ~ 229 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. CGRYRKFVGQLDISIARLKTSMKFVKNVIAGIRETEEKFYYIVQEEKNYRESLTHCRIRGGMLAMPKDEAANTLIADYVAKSGFFRVFIGVNDLEREGQYMFTDNTPLQ

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:28301481).

Specificity

COLEC10 - collectin sub-family member 10 (C-type lectin) (4C1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for COLEC10 Antibody (4C1) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: COLEC10 Antibody (4C1) [H00010584-M01]

Immunocytochemistry/ Immunofluorescence: COLEC10 Antibody (4C1) [H00010584-M01]

COLEC10-Antibody-4C1-Immunocytochemistry-Immunofluorescence-H00010584-M01-img0005.jpg
Immunohistochemistry-Paraffin: COLEC10 Antibody (4C1) [H00010584-M01]

Immunohistochemistry-Paraffin: COLEC10 Antibody (4C1) [H00010584-M01]

COLEC10-Antibody-4C1-Immunohistochemistry-Paraffin-H00010584-M01-img0006.jpg
Immunocytochemistry/ Immunofluorescence: COLEC10 Antibody (4C1) [H00010584-M01]

Immunocytochemistry/ Immunofluorescence: COLEC10 Antibody (4C1) [H00010584-M01]

COLEC10-Antibody-4C1-Immunocytochemistry-Immunofluorescence-H00010584-M01-img0003.jpg
Immunocytochemistry/ Immunofluorescence: COLEC10 Antibody (4C1) [H00010584-M01]

Immunocytochemistry/ Immunofluorescence: COLEC10 Antibody (4C1) [H00010584-M01]

COLEC10-Antibody-4C1-Immunocytochemistry-Immunofluorescence-H00010584-M01-img0004.jpg
Immunohistochemistry: COLEC10 Antibody (4C1) [H00010584-M01]

Immunohistochemistry: COLEC10 Antibody (4C1) [H00010584-M01]

COLEC10-Antibody-4C1-Immunohistochemistry-H00010584-M01-img0002.jpg
Sandwich ELISA: COLEC10 Antibody (4C1) [H00010584-M01] - Detection limit for recombinant GST tagged COLEC10 is 1 ng/ml as a capture antibody.
COLEC10 Antibody (4C1)

Immunohistochemistry-Paraffin: COLEC10 Antibody (4C1) [H00010584-M01] -

Immunohistochemistry-Paraffin: COLEC10 Antibody (4C1) [H00010584-M01] - Cellular & embryonic localisation of CL-L1.A. Immunostaining of ATDC5 cells with the golgi marker 58K & CL-L1. CL-L1 shows localisation with golgi apparatus (white arrow). B. Laminin & CL-L1 coimmunolocalisation. Laminin shows partial cellular immunolocalisiton with CL-L1 around the golgi area (arrows). Scale bar 50 μm C. Laminin & CL-K1 coimmunolocalisation. CL-K1 staining shows a very strong golgi localisation with partial cytoplasmatic laminin colocalisation. D. CL-L1 immunohistochemistry of a 18.5 days postfertilisation mouse embryo. CL-L1 is expressed in the liver (long arrow) & submucosal patal region (short arrows). E. Immunofluorescence showing co-localisation of CL-L1 & Laminin in E13.5 mouse embryos sections. CL-L1 is expressed in the basal membrane of the ephithelium in the palate shelf of the maxilla (arrows). In contrast Laminin expression is present all around the ephitelium membrane. A faint but clear CL-L1 expression is also observed in the cytoplasm of the epithelium & in the mesenchyme of the palate. PSM; rostral extremity of right palatal shelf of maxilla. Scale bar 100 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28301481), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
COLEC10 Antibody (4C1)

Immunocytochemistry/ Immunofluorescence: COLEC10 Antibody (4C1) [H00010584-M01] -

Immunocytochemistry/ Immunofluorescence: COLEC10 Antibody (4C1) [H00010584-M01] - Cellular & embryonic localisation of CL-L1.A. Immunostaining of ATDC5 cells with the golgi marker 58K & CL-L1. CL-L1 shows localisation with golgi apparatus (white arrow). B. Laminin & CL-L1 coimmunolocalisation. Laminin shows partial cellular immunolocalisiton with CL-L1 around the golgi area (arrows). Scale bar 50 μm C. Laminin & CL-K1 coimmunolocalisation. CL-K1 staining shows a very strong golgi localisation with partial cytoplasmatic laminin colocalisation. D. CL-L1 immunohistochemistry of a 18.5 days postfertilisation mouse embryo. CL-L1 is expressed in the liver (long arrow) & submucosal patal region (short arrows). E. Immunofluorescence showing co-localisation of CL-L1 & Laminin in E13.5 mouse embryos sections. CL-L1 is expressed in the basal membrane of the ephithelium in the palate shelf of the maxilla (arrows). In contrast Laminin expression is present all around the ephitelium membrane. A faint but clear CL-L1 expression is also observed in the cytoplasm of the epithelium & in the mesenchyme of the palate. PSM; rostral extremity of right palatal shelf of maxilla. Scale bar 100 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28301481), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
COLEC10 Antibody (4C1)

Immunocytochemistry/ Immunofluorescence: COLEC10 Antibody (4C1) [H00010584-M01] -

Immunocytochemistry/ Immunofluorescence: COLEC10 Antibody (4C1) [H00010584-M01] - Cellular & embryonic localisation of CL-L1.A. Immunostaining of ATDC5 cells with the golgi marker 58K & CL-L1. CL-L1 shows localisation with golgi apparatus (white arrow). B. Laminin & CL-L1 coimmunolocalisation. Laminin shows partial cellular immunolocalisiton with CL-L1 around the golgi area (arrows). Scale bar 50 μm C. Laminin & CL-K1 coimmunolocalisation. CL-K1 staining shows a very strong golgi localisation with partial cytoplasmatic laminin colocalisation. D. CL-L1 immunohistochemistry of a 18.5 days postfertilisation mouse embryo. CL-L1 is expressed in the liver (long arrow) & submucosal patal region (short arrows). E. Immunofluorescence showing co-localisation of CL-L1 & Laminin in E13.5 mouse embryos sections. CL-L1 is expressed in the basal membrane of the ephithelium in the palate shelf of the maxilla (arrows). In contrast Laminin expression is present all around the ephitelium membrane. A faint but clear CL-L1 expression is also observed in the cytoplasm of the epithelium & in the mesenchyme of the palate. PSM; rostral extremity of right palatal shelf of maxilla. Scale bar 100 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28301481), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
COLEC10 Antibody (4C1)

Immunocytochemistry/ Immunofluorescence: COLEC10 Antibody (4C1) [H00010584-M01] -

Immunocytochemistry/ Immunofluorescence: COLEC10 Antibody (4C1) [H00010584-M01] - Cellular & embryonic localisation of CL-L1.A. Immunostaining of ATDC5 cells with the golgi marker 58K & CL-L1. CL-L1 shows localisation with golgi apparatus (white arrow). B. Laminin & CL-L1 coimmunolocalisation. Laminin shows partial cellular immunolocalisiton with CL-L1 around the golgi area (arrows). Scale bar 50 μm C. Laminin & CL-K1 coimmunolocalisation. CL-K1 staining shows a very strong golgi localisation with partial cytoplasmatic laminin colocalisation. D. CL-L1 immunohistochemistry of a 18.5 days postfertilisation mouse embryo. CL-L1 is expressed in the liver (long arrow) & submucosal patal region (short arrows). E. Immunofluorescence showing co-localisation of CL-L1 & Laminin in E13.5 mouse embryos sections. CL-L1 is expressed in the basal membrane of the ephithelium in the palate shelf of the maxilla (arrows). In contrast Laminin expression is present all around the ephitelium membrane. A faint but clear CL-L1 expression is also observed in the cytoplasm of the epithelium & in the mesenchyme of the palate. PSM; rostral extremity of right palatal shelf of maxilla. Scale bar 100 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28301481), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
COLEC10 Antibody (4C1)

Immunocytochemistry/ Immunofluorescence: COLEC10 Antibody (4C1) [H00010584-M01] -

Immunocytochemistry/ Immunofluorescence: COLEC10 Antibody (4C1) [H00010584-M01] - Cellular & embryonic localisation of CL-L1.A. Immunostaining of ATDC5 cells with the golgi marker 58K & CL-L1. CL-L1 shows localisation with golgi apparatus (white arrow). B. Laminin & CL-L1 coimmunolocalisation. Laminin shows partial cellular immunolocalisiton with CL-L1 around the golgi area (arrows). Scale bar 50 μm C. Laminin & CL-K1 coimmunolocalisation. CL-K1 staining shows a very strong golgi localisation with partial cytoplasmatic laminin colocalisation. D. CL-L1 immunohistochemistry of a 18.5 days postfertilisation mouse embryo. CL-L1 is expressed in the liver (long arrow) & submucosal patal region (short arrows). E. Immunofluorescence showing co-localisation of CL-L1 & Laminin in E13.5 mouse embryos sections. CL-L1 is expressed in the basal membrane of the ephithelium in the palate shelf of the maxilla (arrows). In contrast Laminin expression is present all around the ephitelium membrane. A faint but clear CL-L1 expression is also observed in the cytoplasm of the epithelium & in the mesenchyme of the palate. PSM; rostral extremity of right palatal shelf of maxilla. Scale bar 100 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28301481), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for COLEC10 Antibody (4C1) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This product is useful for ELISA. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 28301481).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CL-L1/COLEC10

This gene encodes a member of the C-lectin family, proteins that possess collagen-like sequences and carbohydrate recognition domains. The other members of this family are secreted proteins and bind to carbohydrate antigens on microorganisms facilitating their recognition and removal. This gene product is a cytosolic protein, a characteristic that suggests that it may have different biological functions than other C-lectins. [provided by RefSeq]

Long Name

Collectin Liver 1

Alternate Names

CL-34, COLEC10, Collectin-10, Collectin-34

Entrez Gene IDs

10584 (Human)

Gene Symbol

COLEC10

UniProt

Additional CL-L1/COLEC10 Products

Product Documents for COLEC10 Antibody (4C1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for COLEC10 Antibody (4C1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Citations for COLEC10 Antibody (4C1) - Azide and BSA Free

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Protocols

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