Collagen II Antibody (5B2.5) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80676

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Chicken, Rabbit

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5B2.5

Format

Azide and BSA Free
View all Collagen II Primary Antibodies »

Product Specifications

Immunogen

A purified preparation of lathyritic type II collagen from embryonic chicken sternum

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24349545). Rabbit reactivity reported in scientific literature (PMID: 15621244). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Cartilage matrix

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Collagen II Antibody (5B2.5) - Azide and BSA Free

Immunohistochemistry-Paraffin: Collagen II Antibody (5B2.5) - Azide and BSA Free [NBP2-80676]

Immunohistochemistry-Paraffin: Collagen II Antibody (5B2.5) - Azide and BSA Free [NBP2-80676]

Immunohistochemistry-Paraffin: Collagen II Antibody (5B2.5) - Azide and BSA Free [NBP2-80676] - Analysis of a FFPE section of human cartilage using 1:200 dilution of Collagen II [5B2.5] antibody (NB600-844). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. Image from the standard format of t

Applications for Collagen II Antibody (5B2.5) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

reported in scientific literature (PMID 22558278; 24242000)

Immunohistochemistry-Frozen

reported in scientific literature (PMID 22558278)

Western Blot

1-5ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Collagen II

Highly specific to type II collagen and shows no cross-reaction with types I, III, IV, V, VI, IX, X, or XI. In western blotting, 5B2.5 MAb reacts with the TC fragment of lathritic type II collagen after digestion with mammalian collagenase. It does not react with pepsin-digested type II collagen.

Alternate Names

Chondrocalcin, COL2A1, SEDC

Gene Symbol

COL2A1

Additional Collagen II Products

Product Documents for Collagen II Antibody (5B2.5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Collagen II Antibody (5B2.5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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