Collagen VI alpha 2 Antibody (2C5-F2) - Azide and BSA Free

Novus Biologicals | Catalog # H00001292-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Porcine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2C5-F2

Format

Azide and BSA Free
View all Collagen VI alpha 2 Primary Antibodies »

Product Specifications

Immunogen

COL6A2 (AAH02484, 1 a.a. ~ 425 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTYVRETCGCCDCEKRCGALDVVFVIDSSESIGYTNFTLEKNFVINVVNRLGAIAKDPKSETGTRVGVVQYSHEGTFEAIQLDDEHIDSLSSFKEAVKNLEWIAGGTWTPSALKFAYDRLIKESRRQKTRVFAVVITDGRHDPRDDDLNLRALCDRDVTVTAIGIGDMFHEKHESENLYSIACDKPQQVRNMTLFSDLVAEKFIDDMEDVLCPDPQIVCPDLPCQTELSVAQCTQRPVDIVFLLDGSERLGEQNFHKARRFVEQVARRLTLARRDDDPLNARVALLQFGGPGEQQVAFPLSHNLTAIHEALETTQYLNSFSHVGAGVVHAINAIVRSPRGGARRHAELSFVFLTDGVTGNDSLHESAHSMRKQNVVPTVLALGSDVDMDVLTTLSLGDRAAVFHEKDYDSLAQPGFFDRFIRWIC

Reactivity Notes

Porcine reactivity per customer review.

Specificity

COL6A2 - collagen, type VI, alpha 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Collagen VI alpha 2 Antibody (2C5-F2) - Azide and BSA Free

Western Blot: Collagen VI alpha 2 Antibody (2C5-F2) [H00001292-M01]

Western Blot: Collagen VI alpha 2 Antibody (2C5-F2) [H00001292-M01]

Western Blot: Collagen VI alpha 2 Antibody (2C5-F2) [H00001292-M01] - Analysis of COL6A2 expression in transfected 293T cell line by COL6A2 monoclonal antibody (M01), clone 2C5-F2.Lane 1: COL6A2 transfected lysate(109 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Collagen VI alpha 2 Antibody (2C5-F2) [H00001292-M01]

Immunocytochemistry/ Immunofluorescence: Collagen VI alpha 2 Antibody (2C5-F2) [H00001292-M01]

Immunocytochemistry/Immunofluorescence: Collagen VI alpha 2 Antibody (2C5-F2) [H00001292-M01] - IF staining of Collagen VI alpha 2 in Pig Trabecular meshwork cells using anti-Collagen VI alpha 2 antibody. Image from verified customer review.

Applications for Collagen VI alpha 2 Antibody (2C5-F2) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA. ICC/IF reactivity per customer review.

Reviewed Applications

Read 1 review rated 5 using H00001292-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Collagen VI alpha 2

This gene encodes one of the three alpha chains of type VI collagen, a beaded filament collagen found in most connective tissues. The product of this gene contains several domains similar to von Willebrand Factor type A domains. These domains have been shown to bind extracellular matrix proteins, an interaction that explains the importance of this collagen in organizing matrix components. Mutations in this gene are associated with Bethlem myopathy and Ullrich scleroatonic muscular dystrophy. Three transcript variants have been identified for this gene.

Alternate Names

collagen alpha-2(VI) chain, collagen VI, alpha-2 polypeptide, collagen, type VI, alpha 2,10PP3610, DKFZp586E1322, FLJ46862

Entrez Gene IDs

1292 (Human)

Gene Symbol

COL6A2

OMIM

120 240 158810 254000 (Human)

UniProt

AAH02484 (Human)

Additional Collagen VI alpha 2 Products

Product Documents for Collagen VI alpha 2 Antibody (2C5-F2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Collagen VI alpha 2 Antibody (2C5-F2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Collagen VI alpha 2 Antibody (2C5-F2) - Azide and BSA Free

Customer Reviews for Collagen VI alpha 2 Antibody (2C5-F2) - Azide and BSA Free (1)

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Customer Images

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  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Pig trabecular meshwork cells
    Species: Other
    Verified Customer | Posted 02/02/2016
    IF staining of Collagen VI alpha 2 in Pig Trabecular meshwork cells
    Collagen VI alpha 2 Antibody (2C5-F2) - Azide and BSA Free H00001292-M01

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Protocols

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FAQs

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