Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Feline

Cited:

Human, Mouse, Rat, Feline

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, PCR

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC, PCR

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Collagen VI Primary Antibodies »

Product Specifications

Immunogen

Collagen VI from human and bovine placenta (Uniprot: P12109)

Reactivity Notes

Feline reactivity reported in scientific literature (PMID: 33091431).

Localization

Extracellular matrix.

Specificity

Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Collagen VI alpha 1 and has negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

Store vial at 4C prior to opening. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Collagen VI Antibody - BSA Free

Western Blot: Collagen VI alpha 1 Antibody [NB120-6588]

Western Blot: Collagen VI alpha 1 Antibody [NB120-6588]

Collagen-VI-alpha-1-Antibody-Western-Blot-NB120-6588-img0007.jpg
Immunohistochemistry-Frozen: Collagen VI alpha 1 Antibody [NB120-6588]

Immunohistochemistry-Frozen: Collagen VI alpha 1 Antibody [NB120-6588]

Immunohistochemistry-Frozen: Collagen VI alpha 1 Antibody [NB120-6588] - Feline blood vessels section. The image was captured with an epifluorescent microscope. Image submitted by a verified customer review
Flow Cytometry: Collagen VI alpha 1 Antibody [NB120-6588]

Flow Cytometry: Collagen VI alpha 1 Antibody [NB120-6588]

Collagen-VI-alpha-1-Antibody-Flow-Cytometry-NB120-6588-img0008.jpg
Immunohistochemistry: Collagen VI alpha 1 Antibody [NB120-6588]

Immunohistochemistry: Collagen VI alpha 1 Antibody [NB120-6588]

Immunohistochemistry: Collagen VI alpha 1 Antibody [NB120-6588] - Showed strong staining in FFPE sections of human placenta (Left) with red staining of stromal and extracellular spaces, and in testis (Right) with staining of extracellular spaces between seminiferous tubules). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes for antigen retrieval. Image using the Biotin format of this antibody.
Collagen VI alpha 1 Antibody

Collagen VI alpha 1 Antibody

anti collagen VI antibody ( Lot 26009, 1:400 45 min RT) showed strong staining in FFPE sections of human placenta (Left) with red staining of stromal and extracellular spaces, and in testis (Right) with staining of extracellular spaces between seminiferous tubules). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes for antigen retrieval. Images provided courtesy of LifeSpan Biosciences, Seattle, WA
Collagen VI Antibody - BSA Free

Dot Blot: Collagen VI Antibody - BSA Free [NB120-6588] -

Dot Blot results using Collagen VI Antibody - BSA Free. Sample: A) Human Collagen VI, B) Bovine/Human Collagen VI.Load: 1) 1000ng, 2) 333.33ng, 3) 111.11ng, 4) 37.03ng, 5) 12.35ng.Primary Antibody: Collagen VI Antibody - BSA Free at 5µg/mL at RT for 1hr. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated 1:40,000 at RT for 30mins. Blocking: Blocking Bufferat RT for 30mins.

Applications for Collagen VI Antibody - BSA Free

Application
Recommended Usage

ELISA

1:5000-1:50000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

1:100

Western Blot

1:1000-1:10000
Application Notes
This product has been tested by dot blot and IHC and is suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, and western blotting for highly sensitive qualitative analysis.

Reviewed Applications

Read 1 review rated 4 using NB120-6588 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.

Background: Collagen VI

This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

Gene Symbol

COL6A1

UniProt

P12109 (Human)

Additional Collagen VI Products

Product Documents for Collagen VI Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Collagen VI Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Collagen VI Antibody - BSA Free

Customer Reviews for Collagen VI Antibody - BSA Free (1)

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Customer Images

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  • Collagen VI Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Blood vessels
    Species: Feline
    Verified Customer | Posted 03/09/2018
    Feline blood vessels section. The image was captured with an epifluorescent microscope.
    Collagen VI Antibody - BSA Free NB120-6588

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