Collagen VI Native Protein

Novus Biologicals | Catalog # NBP1-97247

Novus Biologicals
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Key Product Details

Applications

Immunohistochemistry, Western Blot, ELISA
View all Collagen VI Proteins and Enzymes »

Product Specifications

Description

Native Bovine Collagen VI alpha 1

Source: Bovine Placenta (Accession #:AAA98797.1)

Store vial at 4C prior to opening. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use.

Purity

Chromatography

Applications

Immunohistochemistry (1:100-1:500)
Western Blot (1:1000)
ELISA (1:10000)

Application Notes

For use as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation and for other immunological assays as optimized by the user. Specific conditions should be optimized by user.

Protein / Peptide Type

Native Protein

Scientific Data Images for Collagen VI Native Protein

Collagen VI alpha 1 Native Protein Dot Blot: Collagen VI alpha 1 Native Protein [NBP1-97247] -

Dot Blot: Collagen VI alpha 1 Native Protein [NBP1-97247] -

Dot Blot: Collagen VI alpha 1 Native Protein [NBP1-97247] - Dot Blot results using Collagen VI alpha 1 Native Protein. Sample: A) Human Collagen VI, B) Bovine/Human Collagen VI.Load: 1) 1000ng, 2) 333.33ng, 3) 111.11ng, 4) 37.03ng, 5) 12.35ng.Primary Antibody: Rabbit Anti-Collagen VI antibody at 5µg/mL at RT for 1hr. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated 1:40,000 at RT for 30mins. Blocking: BlockOut® Universal Blocking Buffer at RT for 30mins.
Collagen VI Native Protein

Dot Blot: Collagen VI Native Protein [NBP1-97247] -

Dot Blot results using Rabbit Anti-Collagen VI Antibody. Sample: A) Human Collagen VI, B) Bovine/Human Collagen VI.Load: 1) 1000ng, 2) 333.33ng, 3) 111.11ng, 4) 37.03ng, 5) 12.35ng.Primary Antibody: Rabbit Anti-Collagen VI antibody at 5µg/mL at RT for 1hr. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated 1:40,000 at RT for 30mins. Blocking: Blocking Bufferat RT for 30mins.

Formulation, Preparation, and Storage

NBP1-97247
Preparation Method Prepared from Bovine Placenta and is chromatographically and immunologically pure.
Formulation 0.5 M Acetic Acid
Preservative 0.01% Sodium Azide
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C. Do not freeze.

Background: Collagen VI

In muscle tissue, collagen serves as a major component of the endomysium. Collagen constitutes one to two percent of muscle tissue, and accounts for 6% of the weight of strong, tendinous muscles. A collagen may be defined as a protein containing sizable domain(s) of triple-helical conformation. Type IV collagen is a major macromolecular constituent of basement membranes and can be readily isolated from basement-membrane-rich tissues or highly vascularized tissues such as the placental villi. This collagen appears to be largely restricted to structures identifiable as basement membranes. In contrast, type VI collagen appears to be prevalent in several tissues even though it has been isolated largely from placental villi preparations. The extent to which type VII and VIII collagens are distributed is not known.

Gene Symbol

COL6A1

Additional Collagen VI Products

Product Documents for Collagen VI Native Protein

Certificate of Analysis

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Product Specific Notices for Collagen VI Native Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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Protocols

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