Complement C3c Antibody (013E-498.2.3 (10-02A)) - BSA Free

Novus Biologicals | Catalog # NB100-64339

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 013E-498.2.3 (10-02A)

Format

BSA Free
View all Complement C3c Primary Antibodies »

Product Specifications

Immunogen

Human C3 from normal serum, highly purified by chromatography

Specificity

NB100-64339 recognizes 135 kDa complement component 3c (C3c), present in blood serum. C3c is generated over the course of complement activation, where convertase C4b2a (classical pathway) and convertase C3bBb (alternative pathway) cleave C3 to C3b and C3a. C3b is further degraded into iC3b and C3dg/C3d. iC3b is then slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. C3 is crucial in the induction of tolerance generated when an antigen is introduced into immunoprivileged sites and this is exploited by pathogens and cancer cells to evade the immune system by inhibiting complement activation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Complement C3c Antibody (013E-498.2.3 (10-02A)) - BSA Free

Immunohistochemistry-Frozen: Complement C3c Antibody (10-02A) - BSA Free [NB100-64339]

Immunohistochemistry-Frozen: Complement C3c Antibody (10-02A) - BSA Free [NB100-64339]

Immunohistochemistry-Frozen: Complement C3c Antibody (10-02A) [NB100-64339] - Human dermis stained with Complement C3c Antibody. IHC-Fr image submitted by a verified customer review.

Applications for Complement C3c Antibody (013E-498.2.3 (10-02A)) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
In Western Blot binds both C3c and C3 and so is not suitable for all ELISA applications.

Reviewed Applications

Read 1 review rated 5 using NB100-64339 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Borate buffered saline (pH 8.4)

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Complement C3c

The complement factor C3 consists of an alpha and a beta chain. C3 is a central factor in the complement cascade. It is central to the alternative pathway that leads to the C3 convertase C3bBb. The classical mannose binding lectin activation pathway leads to the C3 convertase C4b2a. These convertases cleave C3 resulting in C3a and C3b. Further degradation leads to the formation of the alpha chain products C3d, C3g and C3c. C3 is an acute phase protein that is produced by a wide range of tissues, including renal epithelial cells and hepatocytes.

Alternate Names

AHUS5, ARMD9, ASP, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, complement C3, complement component 3, complement component C3, CPAMD1acylation-stimulating protein cleavage product

Entrez Gene IDs

718 (Human)

Gene Symbol

C3

UniProt

P01024

Additional Complement C3c Products

Product Documents for Complement C3c Antibody (013E-498.2.3 (10-02A)) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Complement C3c Antibody (013E-498.2.3 (10-02A)) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Complement C3c Antibody (013E-498.2.3 (10-02A)) - BSA Free

Customer Reviews for Complement C3c Antibody (013E-498.2.3 (10-02A)) - BSA Free (1)

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  • Complement C3c Antibody (10-02A)
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Dermis
    Species: Human
    Verified Customer | Posted 08/06/2021
    Immunohistochemistry in human dermis
    Complement C3c Antibody (013E-498.2.3 (10-02A)) - BSA Free NB100-64339

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