Complement Component C1qA Antibody (JL-1) - Azide Free

Novus Biologicals | Catalog # NBP1-51139

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunoassay, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Functional

Cited:

Immunohistochemistry-Frozen, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # JL-1

Format

Azide Free
View all Complement Component C1qA Primary Antibodies »

Product Specifications

Immunogen

Purified mouse C1QA

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

Positive Control: Spleen and kidney tissue of wild-type mice (Ref.1) Negative control: Spleen and kidney tissue of C1q -/- mice (Ref.1)

Scientific Data Images for Complement Component C1qA Antibody (JL-1) - Azide Free

Flow Cytometry: Complement Component C1qA Antibody (JL-1) [NBP1-51139]

Flow Cytometry: Complement Component C1qA Antibody (JL-1) [NBP1-51139]

Flow Cytometry: Complement Component C1qA Antibody (JL-1) [NBP1-51139] - Intracellular flow cytometry for C1q in peritoneal macrophages. Anti-C1q was conjugated to PE using a Lightning Link kit for use in flow cytometry. Macrophages are gated on CD45+CD11b+ and T cells are gated on CD45+CD3+ lymphocytes. Dead cells were excluded, and Fc receptors were blocked to prevent non-specific interactions. This is image was submitted via customer review.
Complement Component C1qA Antibody (JL-1) - Azide Free

Western Blot: Complement Component C1qA Antibody (JL-1) - Azide Free [NBP1-51139] -

Validation of Nanostring DEGs with Western blot. Tissue samples from the cortex (A) and hippocampus (B) were processed for Western blot analysis to validate the top DEGs derived from Nanostring. A Expression of the DEG markers PSD95 (Dlg4), NCX1 (Slc8a1), Nefl (Neurofilament Light), and PRAS40 (Akt1s1) in cortex tissue. B Expression of the DEG markers GluN2B (Grin2b), C1qA (C1qa), Nefl, and PKC alpha (Prkca) in the hippocampus. n = 6 mice/group. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs. Sham groups, #p < 0.05, ###p < 0.001 vs. SCI/Male. Two-way ANOVA followed by Tukey’s multiple comparison Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37653491), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Complement Component C1qA Antibody (JL-1) - Azide Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1:100-1:2000

Reviewed Applications

Read 1 review rated 5 using NBP1-51139 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS and 0.1% BSA

Format

Azide Free

Preservative

No Preservative

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Complement Component C1qA

The monoclonal antibody JL-1 recognizes the collagen-like region (CLR) of mouse C1q, a 459 kDa molecule consisting of three individual polypeptide chains. The antibody has been generated by immunization of C1q-/- C57BL/6 mice with purified mouse C1q. C1q forms together with C1r and C1s the C1 macromolecule, the first component of the classical complement pathway. Interaction of immune complexes with C1q induces a conformational change within the C1 complex, which results in activation of the classical pathway. C1q functions as recognition unit by binding to the heavy chain of IgG or IgM (Fc gamma and Fc micro) provided that the immunoglobulins are bound to their antigen. Furthermore, C1q can also recognize molecular patterns associated with pathogens and it can bind to apoptotic blebs, where it activates the classical complement pathway and mediates phagocytosis. As such, C1q promotes the clearance of apoptotic cells and subsequent exposure of auto antigens, thereby preventing stimulation of the immune system. C1q is predominantly produced by macrophages but also by follicular dendritic cells, interdigitating cells and cells of the monocyte-macrophage lineage. C1q deficiency has a profound effect on host defence and clearance of immune complexes. Absence of C1q may cause autoimmunity by impairment of the clearance of apoptotic cells. Inherited C1q deficiency is also associated with the development of systemic lupus erythematosus (SLE). The monoclonal antibody JL-1 is reactive with the collagen-like region (CLR) only, which is the same region to which autoantibodies in mice and humans are binding. Anti-C1q autoantibodies deposit in glomeruli together with C1q but induce overt renal disease only in the context of glomerular immune complex disease. This provides an explanation why anti-C1q antibodies are especially pathogenic in patients with SLE.

Long Name

Complement Component 1q Subcomponent, A Chain

Alternate Names

C1QA

Gene Symbol

C1QA

Additional Complement Component C1qA Products

Product Documents for Complement Component C1qA Antibody (JL-1) - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Complement Component C1qA Antibody (JL-1) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Complement Component C1qA Antibody (JL-1) - Azide Free

Customer Reviews for Complement Component C1qA Antibody (JL-1) - Azide Free (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Complement Component C1qA Antibody (JL-1) - Azide Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Complement Component C1qA Antibody (JL-1)
    Name: Oliver Burton
    Application: Flow Cytometry
    Sample Tested: Peritoneal macrophages
    Species: Mouse
    Verified Customer | Posted 03/08/2018
    Anti-C1q was conjugated to PE using a Lightning Link kit for use in flow cytometry. Macrophages are gated on CD45+CD11b+ and T cells are gated on CD45+CD3+ lymphocytes. Dead cells were excluded, and Fc receptors were blocked to prevent non-specific interactions.
    Complement Component C1qA Antibody (JL-1) - Azide Free NBP1-51139

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies