Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 32-G1

Format

Azide and BSA Free
View all Complement Component C5aR1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was raised by immunizing mice a synthetic Complement Component C5aR1 C-terminal phosphopeptide that contained phosphoserine instead of serine at position 334 and also at the neighboring phosphorylation sites Ser-332 and Ser-338.

Specificity

The 32-G1 antibody recognizes Complement Component C5aR1 which has its serine 334 phosphorylated. The mAb 32-G1 reacted specifically with a Complement Component C5aR1 peptide that contained phosphoserine 334 and also reacted with Complement Component C5aR1s that were isolated from C5a-stimulated, transfected RBL-Complement Component C5aR1 cells.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Novus Biologicals Rabbit Complement Component C5aR1 Antibody (32-G1) - Chimeric - Azide and BSA Free (NBP3-12085) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images

Immunocytochemistry/ Immunofluorescence: Complement Component C5aR1 Antibody (32-G1) - Chimeric - Azide and BSA Free [NBP3-12085]

Immunocytochemistry/ Immunofluorescence: Complement Component C5aR1 Antibody (32-G1) - Chimeric - Azide and BSA Free [NBP3-12085]

Immunocytochemistry/Immunofluorescence: Complement Component C5aR1 Antibody (32-G1) - Chimeric [NBP3-12085] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version 32-G1 (NBP3-12085) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12085, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.
Complement Component C5aR1 Antibody (32-G1) - Chimeric - Azide and BSA Free

Flow Cytometry: Complement Component C5aR1 Antibody (32-G1) - Chimeric - Azide and BSA Free [NBP3-12085] -

Flow Cytometry: Complement Component C5aR1 Antibody (32-G1) - Chimeric - Azide and BSA Free [NBP3-12085] - U937 cells were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12085 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Applications

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: Complement Component C5aR1

The human anaphylatoxin C5a is a 74 residue glycopeptide which is generated by proteolytic cleavage of the complement factor C5 in the course of complement activation. A variety of biological effects evoked by C5a can be demonstrated, rendering this molecule an important mediartor of inflammation, with granulocytes and macrophages as the main target cells. All cellular responses to C5a are specifically mediated by interactions with the membrane bound C5a receptor, a seven transmembrane GTP binding coupled receptor that belongs to the rhodopsin supergene family. C5a receptor 1 expression has been reported in myeloid blood cells, brain, liver, lung, spleen, heart, kidney, and intestinal tract. ESTs have been isolated from B cell/lung/testis, nerve, and placenta libraries.

Long Name

Complement Component C5a Receptor 1

Alternate Names

C5AR1, CD88, Complement Component C5a R1

Gene Symbol

C5AR1

Additional Complement Component C5aR1 Products

Product Documents

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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