Complement Component C9 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15952

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Complement Component C9. The exact sequence is proprietary.

Localization

Membrane; Multi-pass membrane protein; Secreted; Membrane; Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Complement Component C9 Antibody - BSA Free (NBP2-15952) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Complement Component C9 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Complement Component C9 Antibody - BSA Free

Immunohistochemistry: Complement Component C9 Antibody [NBP2-15952]

Immunohistochemistry: Complement Component C9 Antibody [NBP2-15952]

Complement-Component-C9-Antibody-Immunohistochemistry-NBP2-15952-img0010.jpg
Western Blot: Complement Component C9 Antibody [NBP2-15952]

Western Blot: Complement Component C9 Antibody [NBP2-15952]

Western Blot: Complement Component C9 Antibody [NBP2-15952] - Sample (30 ug of whole cell lysate) A: THP-1 7. 5% SDS PAGE gel, diluted at 1:500.
Western Blot: Complement Component C9 Antibody [NBP2-15952]

Western Blot: Complement Component C9 Antibody [NBP2-15952]

Western Blot: Complement Component C9 Antibody [NBP2-15952] - Human plasma (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C9 antibody [N2C2-2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: Complement Component C9 Antibody [NBP2-15952]

Western Blot: Complement Component C9 Antibody [NBP2-15952]

Western Blot: Complement Component C9 Antibody [NBP2-15952] - Rat plasma (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Complement Component C9 Antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Complement Component C9 Antibody [NBP2-15952]

Western Blot: Complement Component C9 Antibody [NBP2-15952]

Western Blot: Complement Component C9 Antibody [NBP2-15952] - Mouse plasma (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Complement Component 9 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody,
Complement Component C9 Antibody

Immunocytochemistry/ Immunofluorescence: Complement Component C9 Antibody [NBP2-15952] -

Immunocytochemistry/ Immunofluorescence: Complement Component C9 Antibody [NBP2-15952] - Staining of vascular BM whole mounts with antibodies to proteins detected in the proteome analysis.A generic staining of the vascular BMs was given by an antibody to the 7S domain of collagen IV alpha 3 (A, C, E, F, G). Prominent staining for microvascular aneurisms was detected by staining with antibodies to C9 (B, C), Fibronectin (FN, E), ApoE (F) & PRELP (G). The same treatment of vascular BM whole mounts from non-diabetic eyes did not show staining for these proteins (D). A norrin-specific staining is shown to be generic for the entire vascular BM whole mounts (H), the signal, however, being less prominent in vascular aneurisms (arrow in H). Staining of vascular BM whole mounts from non-diabetic eyes showed a clearly weaker staining for norrin, when compared to vascular whole mounts from non-diabetic donors. Bar: 25um. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0189857), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Complement Component C9 Antibody

Immunocytochemistry/ Immunofluorescence: Complement Component C9 Antibody [NBP2-15952] -

Immunocytochemistry/ Immunofluorescence: Complement Component C9 Antibody [NBP2-15952] - Staining of vascular BM whole mounts with antibodies to proteins detected in the proteome analysis.A generic staining of the vascular BMs was given by an antibody to the 7S domain of collagen IV alpha 3 (A, C, E, F, G). Prominent staining for microvascular aneurisms was detected by staining with antibodies to C9 (B, C), Fibronectin (FN, E), ApoE (F) & PRELP (G). The same treatment of vascular BM whole mounts from non-diabetic eyes did not show staining for these proteins (D). A norrin-specific staining is shown to be generic for the entire vascular BM whole mounts (H), the signal, however, being less prominent in vascular aneurisms (arrow in H). Staining of vascular BM whole mounts from non-diabetic eyes showed a clearly weaker staining for norrin, when compared to vascular whole mounts from non-diabetic donors. Bar: 25um. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0189857), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Complement Component C9 Antibody

Immunocytochemistry/ Immunofluorescence: Complement Component C9 Antibody [NBP2-15952] -

Immunocytochemistry/ Immunofluorescence: Complement Component C9 Antibody [NBP2-15952] - Staining of vascular BM whole mounts with antibodies to proteins detected in the proteome analysis.A generic staining of the vascular BMs was given by an antibody to the 7S domain of collagen IV alpha 3 (A, C, E, F, G). Prominent staining for microvascular aneurisms was detected by staining with antibodies to C9 (B, C), Fibronectin (FN, E), ApoE (F) & PRELP (G). The same treatment of vascular BM whole mounts from non-diabetic eyes did not show staining for these proteins (D). A norrin-specific staining is shown to be generic for the entire vascular BM whole mounts (H), the signal, however, being less prominent in vascular aneurisms (arrow in H). Staining of vascular BM whole mounts from non-diabetic eyes showed a clearly weaker staining for norrin, when compared to vascular whole mounts from non-diabetic donors. Bar: 25um. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0189857), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Complement Component C9 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Complement Component C9 Antibody [NBP2-15952] -

C9 antibody [N2C2-2], Internal detects C9 protein at by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: C9 stained by C9 antibody [N2C2-2], Internal (NBP2-15952) diluted at 1:500.
Blue: Hoechst 33342 staining.

Applications for Complement Component C9 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Complement Component C9

This gene encodes the final component of the complement system. It participates in the formation of the Membrane Attack Complex (MAC). The MAC assembles on bacterial membranes to form a pore, permitting disruption of bacterial membrane organization. Mutations in this gene cause component C9 deficiency. [provided by RefSeq]

Alternate Names

complement component 9, complement component C9

Gene Symbol

C9

UniProt

Additional Complement Component C9 Products

Product Documents for Complement Component C9 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Complement Component C9 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for Complement Component C9 Antibody - BSA Free

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Protocols

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