COMT Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15954

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Rat

Predicted:

Rhesus Macaque (94%). Backed by our 100% Guarantee.

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all COMT Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human COMT. The exact sequence is proprietary.

Reactivity Notes

Mouse (82%), Dog (81%), Pig (85%), Bovine (83%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Knockout (KO) Validated Rabbit COMT Antibody - BSA Free (NBP2-15954) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for COMT Antibody - BSA Free

Western Blot: COMT Antibody [NBP2-15954]

Western Blot: COMT Antibody [NBP2-15954]

Western Blot: COMT Antibody [NBP2-15954] - Wild-type (WT) and COMT knockout (KO) HEK293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with COMT antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: COMT Antibody [NBP2-15954]

Western Blot: COMT Antibody [NBP2-15954]

Western Blot: COMT Antibody [NBP2-15954] - COMT antibody detects COMT protein by Western blot analysis. A. 30 ug K562 whole cell lysate/extract 12 % SDS-PAGECOMT antibody dilution: 1:1000.
Immunohistochemistry-Paraffin: COMT Antibody [NBP2-15954]

Immunohistochemistry-Paraffin: COMT Antibody [NBP2-15954]

Immunohistochemistry-Paraffin: COMT Antibody [NBP2-15954] - Immunohistochemical analysis of paraffin-embedded DLD1 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: COMT Antibody [NBP2-15954]

Immunohistochemistry-Paraffin: COMT Antibody [NBP2-15954]

Immunohistochemistry-Paraffin: COMT Antibody [NBP2-15954] - Paraffin-embedded Rat brain. COMT antibody diluted at 1:500.
COMT Antibody - BSA Free

Immunohistochemistry-Paraffin: COMT Antibody [NBP2-15954] -

COMT antibody detects COMT protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
COMT antibody (NBP2-15954) diluted at 1:500.
br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for COMT Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: COMT

Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq]

Long Name

Catechol O-Methyltransferase

Alternate Names

catechol O-methyltransferase, catechol-O-methyltransferase, EC 2.1.1.6

Gene Symbol

COMT

UniProt

P21964 (Human)

Additional COMT Products

Product Documents for COMT Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for COMT Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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