Connexin 43/GJA1 Antibody (M496)

Name: Connexin 43/GJA1 Antibody (M496)
Brand: Novus Biologicals
SKU: NBP3-23240
Price: 429 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-23240

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat, Chicken

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # M496

View all Connexin 43/GJA1 Primary Antibodies »

Product Specifications

Immunogen

Clone M496 was generated from a peptide fragment in the C-terminal region of human Connexin 43/GJA1. This sequence is highly conserved in mouse and rat Connexin 43/GJA1. [UniProt# P17302]

Specificity

The antibody detects a 43 kDa protein corresponding to the apparent molecular mass of Connexin 43/GJA1 on SDS-PAGE immunoblots of mouse brain. The antibody is also useful for immunofluorescent labeling of gap junctions in cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for Connexin 43/GJA1 Antibody (M496)

Western Blot: Connexin 43/GJA1 Antibody (M496) [NBP3-23240] -

Western Blot: Connexin 43/GJA1 Antibody (M496) [NBP3-23240] - Western blot analysis of Connexin 43/GJA1 expression in adult mouse brain (lanes 1-3). The blots were probed with mouse monoclonal anti-Connexin 43/GJA1 at 1:250 (lane 1), 1:500 (lane 2), and 1:1000 (lane 3).

Applications for Connexin 43/GJA1 Antibody (M496)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Connexin 43/GJA1

Connexin 43/GJA1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. GJA1 is the major cardiac gap junction protein, which is thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. GJA1 is targeted by several protein kinases that regulate myocardial cell cell coupling. A related intronless GJA1 pseudogene, GJA1P, has been mapped to chromosome 5.

Alternate Names

CX43, GJA1, GJAL, ODDD, ODOD, SDTY3

Gene Symbol

GJA1

Additional Connexin 43/GJA1 Products

Product Documents for Connexin 43/GJA1 Antibody (M496)

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Certificate of Analysis

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Product Specific Notices for Connexin 43/GJA1 Antibody (M496)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.