COP9 Antibody (2D10) - Azide and BSA Free

Novus Biologicals | Catalog # H00008533-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2D10

Format

Azide and BSA Free
View all COP9 Primary Antibodies »

Product Specifications

Immunogen

COPS3 (NP_003644, 324 a.a. ~ 422 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SRVQLSGPQEAEKYVLHMIEDGEIFASINQKDGMVSFHDNPEKYNNPAMLHNIDQEMLKCIELDERLKAMDQEITVNPQFVQKSMGSQEDDSGNKPSSY

Localization

Cytoplasmic and Nuclear

Specificity

COPS3 - COP9 constitutive photomorphogenic homolog subunit 3 (Arabidopsis)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for COP9 Antibody (2D10) - Azide and BSA Free

Western Blot: COP9 Antibody (2D10) [H00008533-M02]

Western Blot: COP9 Antibody (2D10) [H00008533-M02]

Western Blot: COP9 Antibody (2D10) [H00008533-M02] - COPS3 monoclonal antibody (M02), clone 2D10. Analysis of COPS3 expression in PC-12.
Immunocytochemistry/ Immunofluorescence: COP9 Antibody (2D10) [H00008533-M02]

Immunocytochemistry/ Immunofluorescence: COP9 Antibody (2D10) [H00008533-M02]

Immunocytochemistry/Immunofluorescence: COP9 Antibody (2D10) [H00008533-M02] - Analysis of monoclonal antibody to COPS3 on HeLa cell. Antibody concentration 20 ug/ml.
Western Blot: COP9 Antibody (2D10) [H00008533-M02]

Western Blot: COP9 Antibody (2D10) [H00008533-M02]

Western Blot: COP9 Antibody (2D10) [H00008533-M02] - COPS3 monoclonal antibody (M02), clone 2D10 Analysis of COPS3 expression in HeLa.
Western Blot: COP9 Antibody (2D10) [H00008533-M02]

Western Blot: COP9 Antibody (2D10) [H00008533-M02]

Western Blot: COP9 Antibody (2D10) [H00008533-M02] - Analysis of COPS3 expression in transfected 293T cell line by COPS3 monoclonal antibody (M02), clone 2D10.Lane 1: COPS3 transfected lysate(47.9 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: COP9 Antibody (2D10) [H00008533-M02] - Detection limit for recombinant GST tagged COPS3 is approximately 0.1ng/ml as a capture antibody.

Applications for COP9 Antibody (2D10) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: COP9

The protein encoded by this gene possesses kinase activity that phosphorylates regulators involved in signal transduction. It phosphorylates I kappa-Balpha, p105, and c-Jun. It acts as a docking site for complex-mediated phosphorylation. The gene is located within the Smith-Magenis syndrome region on chromosome 17.

Alternate Names

COP9 constitutive photomorphogenic homolog subunit 3 (Arabidopsis), COP9 signalosome complex subunit 3, CSN3COP9 complex subunit 3, JAB1-containing signalosome subunit 3, SGN3COP9 (constitutive photomorphogenic, Arabidopsis, homolog) subunit 3, Signalosome subunit 3

Entrez Gene IDs

8533 (Human)

Gene Symbol

COPS3

OMIM

604665 (Human)

UniProt

NP_003644 (Human)

Additional COP9 Products

Product Documents for COP9 Antibody (2D10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for COP9 Antibody (2D10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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