COPG2 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-92869

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 542-871 of human COPG2 (NP_036265.3). LNATYIFNGLTVSVPGMEKALHQYTLEPSEKPFDMKSIPLAMAPVFEQKAEITLVATKPEKLAPSRQDIFQEQLAAIPEFLNIGPLFKSSEPVQLTEAETEYFVRCIKHMFTNHIVFQFDCTNTLNDQLLEKVTVQMEPSDSYEVLSCIPAPSLPYNQPGICYTLVRLPDDDPTAVAGSFSCTMKFTVRDCDPNTGVPDEDGYDDEYVLEDLEVTVSDHIQKVLKPNFAAAWEEVGDTFEKEETFALSSTKTLEEAVNNIITFLGMQPCERSDKVPENKNSHSLYLAGIFRGGYDLLVRSRLALADGVTMQVTVRSKERTPVDVILASVG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for COPG2 Antibody - Azide and BSA Free

Western Blot: COPG2 AntibodyAzide and BSA Free [NBP2-92869]

Western Blot: COPG2 AntibodyAzide and BSA Free [NBP2-92869]

Western Blot: COPG2 Antibody [NBP2-92869] - Analysis of extracts of various cell lines, using COPG2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: COPG2 Antibody - Azide and BSA Free [NBP2-92869]

Immunocytochemistry/ Immunofluorescence: COPG2 Antibody - Azide and BSA Free [NBP2-92869]

Immunocytochemistry/Immunofluorescence: COPG2 Antibody [NBP2-92869] - Analysis of U-2 OS cells using COPG2. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: COPG2 Antibody - Azide and BSA Free [NBP2-92869]

Immunohistochemistry-Paraffin: COPG2 Antibody - Azide and BSA Free [NBP2-92869]

Immunohistochemistry-Paraffin: COPG2 Antibody [NBP2-92869] - Paraffin-embedded mouse brain using COPG2.

Applications for COPG2 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:50-1:100

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: COPG2

Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two Coatomer-Protein mechanisms (COPI and COPII) and although they have mechanistic parallels, they are molecularly distinct. The COPI coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COPI) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COPI, and ARF. COPI has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COPII has been shown to be independently and selectively recruited to the ER relative to COPI subunits.

Alternate Names

2-COP, coat protein, nonclathrin, gamma-2-cop, coatomer protein complex, subunit gamma 2, coatomer subunit gamma-2, DKFZp761N09121, FLJ11781, Gamma-2-coat protein, Gamma-2-COP

Gene Symbol

COPG2

Additional COPG2 Products

Product Documents for COPG2 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for COPG2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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FAQs

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