Cortactin Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15971

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cortactin Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Cortactin. The exact sequence is proprietary.

Localization

Cytoplasm; cytoskeleton (By similarity); Cell projection; lamellipodium (By similarity); ruffle (By similarity)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cortactin Antibody - BSA Free

Knockout Validated: Cortactin Antibody [NBP2-15971]

Western Blot: Cortactin Antibody [NBP2-15971]

Western Blot: Cortactin Antibody [NBP2-15971] - Wild-type (WT) and Cortactin knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1], N-term. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Cortactin Antibody [NBP2-15971]

Western Blot: Cortactin Antibody [NBP2-15971]

Western Blot: Cortactin Antibody [NBP2-15971] - Sample (30 ug of whole cell lysate) A: PC-12 7. 5% SDS PAGE gel, diluted at 1:3000.
Immunohistochemistry-Paraffin: Cortactin Antibody [NBP2-15971]

Immunohistochemistry-Paraffin: Cortactin Antibody [NBP2-15971]

Immunohistochemistry-Paraffin: Cortactin Antibody [NBP2-15971] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.
Western Blot: Cortactin Antibody [NBP2-15971]

Western Blot: Cortactin Antibody [NBP2-15971]

Western Blot: Cortactin Antibody [NBP2-15971] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7. 5% SDS PAGE gel, diluted at 1:1000.
Cortactin Antibody

Immunohistochemistry-Paraffin: Cortactin Antibody [NBP2-15971] -

Immunohistochemistry-Paraffin: Cortactin Antibody [NBP2-15971] - Cortactin antibody [N1], N-term detects Cortactin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Cortactin stained by Cortactin antibody [N1], N-term (NBP2-15971) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Cortactin Antibody

Immunocytochemistry/ Immunofluorescence: Cortactin Antibody [NBP2-15971] -

Immunocytochemistry/ Immunofluorescence: Cortactin Antibody [NBP2-15971] - Cortactin antibody [N1], N-term detects Cortactin protein at cytoplasm and cell membrane by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cortactin stained by Cortactin antibody [N1], N-term (NBP2-15971) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.
Scale bar= 10um.
Cortactin Antibody

Western Blot: Cortactin Antibody [NBP2-15971] -

Western Blot: Cortactin Antibody [NBP2-15971] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1], N-term (NBP2-15971) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Cortactin Antibody

Western Blot: Cortactin Antibody [NBP2-15971] -

Western Blot: Cortactin Antibody [NBP2-15971] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1], N-term (NBP2-15971) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Cortactin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cortactin

This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq]

Alternate Names

Amplaxin, CTTN, EMS1

Gene Symbol

CTTN

UniProt

Q14247 (Human)

Additional Cortactin Products

Product Documents for Cortactin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Cortactin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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