COX-2 Antibody

Novus Biologicals | Catalog # NB100-689

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Simple Western

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all COX-2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein corresponding to the C-terminus of rat COX2 (the exact amino acid sequence is considered to be proprietary information).

Localization

Microsomal membrane, cytoplasmic

Specificity

This reacts with the 70 kDa protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit COX-2 Antibody (NB100-689) is a polyclonal antibody validated for use in IHC, WB and Simple Western. Anti-COX-2 Antibody: Cited in 35 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for COX-2 Antibody

Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-689]

Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-689]

Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-689] - Formalin fixed paraffin embedded colon carcinoma stained with COX-2 antibody (NB100-689).
Simple Western: COX-2 Antibody [NB100-689]

Simple Western: COX-2 Antibody [NB100-689]

Simple Western: COX-2 Antibody [NB100-689] - Simple Western lane view shows a specific band for COX2 in 1.0 mg/ml of Rat Brain at an antibody concentration of 1:100. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image provided through customer review.
COX-2 Antibody

Western Blot: COX-2 Antibody [NB100-689] -

(A) The COX-2 level in U-118 MG, BJ, and HaCaT cells after 24 h treatment with CXB, FL, or CXB+FL mixture at non-toxic concentrations and IC50. Results are presented as medians expressed as percent of non-treated control. The whiskers are lower (25%) and upper (75%) quartile ranges. Symbol * shows statistically significant difference against control, p < 0.05, Kruskal–Wallis test. (B) Image of immunoblots. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/25/6/3226), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
COX-2 Antibody

Western Blot: COX-2 Antibody [NB100-689] -

Effects of Rut on the expression of cyclooxygenase-2 (COX-2), interleukin-1 beta (IL-1 beta ), and inducible nitric oxide synthase (iNOS) in LTA-stimulated RAW cells. (A–D) Cells were pretreated with Rut (10 and 20 μM) for 20 min and then stimulated by LTA (10 μg/mL) for 24 h. The levels of (B) COX-2 (C) IL-1 beta and (D) iNOS protein expression were evaluated as described in the Methods section. Data are presented as the means +/- SEM (n = 4); * p < 0.05, ** p < 0.01, and *** p < 0.001, compared with the control group; # p < 0.05 and ## p < 0.01, compared with the LTA group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35682568), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for COX-2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Simple Western

1:100

Western Blot

1:100-1:2000
Application Notes

IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 31592103).
See Simple Western Antibody Database for Simple Western validation: Tested in Rat Brain, separated by Size, antibody dilution of 1:100, apparent MW was ~55-66 kDa

Reviewed Applications

Read 1 review rated 2 using NB100-689 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 0.2% BSA, Tween-20

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: COX-2

COX2 (Cyclooxygenase 2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, COX2 is therefore of interest in studying immune response regulation. COX2 is induced by a wide variety of stimuli and was initally identified as immediate early growth response gene. In addition, COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged.

Long Name

Cyclooxygenase 2

Alternate Names

COX2, PGHS-2, PHS-II, PTGS2

Entrez Gene IDs

5743 (Human); 19225 (Mouse); 29527 (Rat)

Gene Symbol

PTGS2

UniProt

P35355 (Rat)

Additional COX-2 Products

Product Documents for COX-2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for COX-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for COX-2 Antibody (1)

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Customer Images

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  • Name: Anonymous
    Application: Simple Western
    Sample Tested: Non membrane associated protein lysate from rat brains
    Species: Rat
    Verified Customer | Posted 02/19/2015
    COX-2 Antibody NB100-689

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Protocols

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FAQs for COX-2 Antibody

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  • Q: Can you please tell me the concentration for product NB100-689 (Lot #Y295)?

    A: The concentration for the lot# Y295 of NB100-689 (COX-2 Antibody) is 0.08 mg/ml.

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