COX-2 Antibody

Novus Biologicals | Catalog # NB110-1948

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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit Serum
View all COX-2 Primary Antibodies »

Product Specifications

Immunogen

This whole rabbit serum was prepared by repeated immunizations with a fusion protein corresponding to the carboxy-terminus of human COX-2. (Uniprot: P35354)

Reactivity Notes

Reactivity against homologues from other sources is not known

Localization

Membrane associated. Microsomal membrane.

Specificity

This antiserum is directed against human COX-2. Two different isoforms of the enzyme are known, Cox-1 and COX-2. A BLAST analysis was used to suggest cross-reactivity with COX-2 in human, mouse and rat based on a homology with the immunizing sequence and should not cross react with Cox-1. Reactivity against homologues from other sources is not known. Reactivity of this antibody with COX-2 from other species is unknown

Clonality

Polyclonal

Host

Rabbit

Isotype

Serum

Description

This antiserum is directed against human Cox-2

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for COX-2 Antibody

Western Blot: COX-2 Antibody [NB110-1948]

Western Blot: COX-2 Antibody [NB110-1948]

Western Blot: COX-2 Antibody [NB110-1948] - COX-2 antibody is shown to detect COX-2 present in COX-2 transfected Sf9 cell extract (lane 1) and IL-1beta induced WISH cell extract (lane 2). Detection occurs using a 1:10,000 dilution of antibody followed by 1:10,000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1'. Other detection systems will yield similar results.
COX-2 Antibody

COX-2 Antibody

anti-Cox2 is shown to detect Cox-2 present in Cox-2 transfected Sf9 cell extract (lane 1) and IL-1beta induced WISH cell extract (lane 2). Detection occurs using a 1:10,000 dilution of antibody followed by 1:10,000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1'. Other detection systems will yield similar results.

Applications for COX-2 Antibody

Application
Recommended Usage

ELISA

1:10000-1:50000

Immunohistochemistry

1:10-1:500

Western Blot

1:5000-1:10000
Application Notes
Cox-2 (Cyclooxygenase-2) is an inducible enzyme that is normally absent from cells, however, in response to growth factors, tumor promoters and some cytokines, it undergoes a rapid and transient expression. This antiserum against Human Cox-2 and has been tested for use in immunoblotting. The antibody recognizes Cox-2 at 70kDa in Sf9 cell lines transfected with Cox-2 as well as in WISH cells induced with IL-1b. Reactivity in other immunoassays is unknown.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Antiserum

Preservative

0.01% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: COX-2

Cox-2 (Cyclooxygenase-2) is an inducible enzyme that is normally absent from cells, however, in response to growth factors, tumor promoters and some cytokines, it undergoes a rapid and transient expression. The antibody recognizes Cox-2 at 70kDa in Sf9 cell lines transfected with Cox-2 as well as in WISH cells induced with IL-1b.

Long Name

Cyclooxygenase 2

Alternate Names

COX2, PGHS-2, PHS-II, PTGS2

Entrez Gene IDs

5743 (Human); 19225 (Mouse); 29527 (Rat)

Gene Symbol

PTGS2

UniProt

P35354 (Human)

Additional COX-2 Products

Product Documents for COX-2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for COX-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for COX-2 Antibody

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  • Q: Has it been tested in IHC-frozen? A customer is looking for an anti Cox-2 which is suitable for frozen sections with rat tissue.

    A: This hasn't been tested in IHC-Fr however the chances of it working are high as it has been tested with IHC-P. The customer may like to test this as part of our Innovators Reward Program. The only COX2 antibody we have tested in IHC-Fr is NBP1-50486, but we have not tested this in rat. Your customer can also apply for Innovator's for this Ab to test in rat. The % homology is 86% and our lab recommends use if the homology is 85% or above.

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