COX-2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85499

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all COX-2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RQMKYQSFNEYRKRFMLKPYESFEELTGEKEMSAELEALYGDIDAVELYPALLVEKPRPDAIFGETMVEVGAPFSLKGLMGNVICSPAYWKPSTFGGEVGFQIINTASIQSLICNNVKGCPFTSFSVPDPE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (88%). Human reactivity reported in scientific literature (PMID: 23090667).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for COX-2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: COX-2 Antibody [NBP1-85499]

Immunocytochemistry/ Immunofluorescence: COX-2 Antibody [NBP1-85499]

Immunocytochemistry/Immunofluorescence: COX-2 Antibody [NBP1-85499] - Staining of human cell line A549 shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: COX-2 Antibody [NBP1-85499]

Immunohistochemistry-Paraffin: COX-2 Antibody [NBP1-85499]

Immunohistochemistry-Paraffin: COX-2 Antibody [NBP1-85499] - Staining in human urinary bladder shows strong cytoplasmic positivity in urothelial cells.
Immunohistochemistry-Paraffin: COX-2 Antibody [NBP1-85499]

Immunohistochemistry-Paraffin: COX-2 Antibody [NBP1-85499]

Immunohistochemistry-Paraffin: COX-2 Antibody [NBP1-85499] - Staining of human skeletal muscle shows no postivity in myocytes as expected.
Immunohistochemistry-Paraffin: COX-2 Antibody [NBP1-85499]

Immunohistochemistry-Paraffin: COX-2 Antibody [NBP1-85499]

Immunohistochemistry-Paraffin: COX-2 Antibody [NBP1-85499] - Staining of human kidney shows not postivity in cells in tubules and cells in glomeruli as expected.
Immunohistochemistry-Paraffin: COX-2 Antibody [NBP1-85499]

Immunohistochemistry-Paraffin: COX-2 Antibody [NBP1-85499]

Immunohistochemistry-Paraffin: COX-2 Antibody [NBP1-85499] - Staining of human seminal vesicle shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: COX-2 Antibody [NBP1-85499]

Immunohistochemistry-Paraffin: COX-2 Antibody [NBP1-85499]

Immunohistochemistry-Paraffin: COX-2 Antibody [NBP1-85499] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.

Applications for COX-2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: COX-2

COX2 (Cyclooxygenase 2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, COX2 is therefore of interest in studying immune response regulation. COX2 is induced by a wide variety of stimuli and was initially identified as immediate early growth response gene. In addition, COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged.

Long Name

Cyclooxygenase 2

Alternate Names

COX2, PGHS-2, PHS-II, PTGS2

Gene Symbol

PTGS2

Additional COX-2 Products

Product Documents for COX-2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for COX-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for COX-2 Antibody - BSA Free

Customer Reviews for COX-2 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review COX-2 Antibody - BSA Free and earn rewards!

Have you used COX-2 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for COX-2 Antibody - BSA Free

Showing  1 - 1 of 1 FAQ Showing All

  • Q: I am a student researching COX-2 expression in felines and am looking for a suitable primary antibody to use in IHC. To your knowledge, have any of your COX-2 antibodies been used successfully with feline COX-2? If yes, I would be very interested in learning more. If not, would it be possible to run a sequence homology of feline COX-2 (GenBank ABM65700.1, accession EF036473.1) against your available COX-2 antibodies to determine the overlap and likelihood of cross-reactivity?

    A: We have not tested any of these antibodies for their reactivity in felines. However, we do have a number of antibodies that look like they may cross-react with feline. NBP1-85499 recognizes a region that is about 90-91% similar to feline COX2. NBP2-04015 recognizes a region in humans that is 94% similar to feline COX2. NB100-868 recognizes a region in humans at the far C-terminal 12 amino acids that is 100% identical to feline COX2. NBP1-19354 recognizes a region that is also about 95% similar to feline COX2. Only NBP1-85499 and NBP1-19354 have been tested for use in IHC. If you would like to test this antibody in an untested species and/or application and share your results with us, then I can recommend our Innovators Reward Program.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies