COX-2 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-85499
![Immunocytochemistry/ Immunofluorescence: COX-2 Antibody [NBP1-85499]](https://resources.rndsystems.com/images/products/COX-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85499-img0004.jpg "Immunocytochemistry/ Immunofluorescence: COX-2 Antibody [NBP1-85499]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Scientific Data Images for COX-2 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: COX-2 Antibody [NBP1-85499]
Immunocytochemistry/Immunofluorescence: COX-2 Antibody [NBP1-85499] - Staining of human cell line A549 shows localization to endoplasmic reticulum. Antibody staining is shown in green.![COX-2 Antibody - BSA Free Immunohistochemistry-Paraffin: COX-2 Antibody [NBP1-85499]](https://resources.rndsystems.com/images/products/nbp1-85499_-immunohistochemistry-paraffin-639173123161086845.jpg "Immunohistochemistry-Paraffin: COX-2 Antibody [NBP1-85499]")
Immunohistochemistry-Paraffin: COX-2 Antibody [NBP1-85499]
Staining of human skeletal muscle shows no positivity in myocytes as expected.![COX-2 Antibody - BSA Free Immunohistochemistry-Paraffin: COX-2 Antibody [NBP1-85499]](https://resources.rndsystems.com/images/products/nbp1-85499_-immunohistochemistry-paraffin-639173123462223635.jpg "Immunohistochemistry-Paraffin: COX-2 Antibody [NBP1-85499]")
Immunohistochemistry-Paraffin: COX-2 Antibody [NBP1-85499]
Staining of human testis shows no positivity in cells in seminiferous ducts as expected.![COX-2 Antibody - BSA Free Immunohistochemistry-Paraffin: COX-2 Antibody [NBP1-85499]](https://resources.rndsystems.com/images/products/nbp1-85499_-immunohistochemistry-paraffin-639173123661198727.jpg "Immunohistochemistry-Paraffin: COX-2 Antibody [NBP1-85499]")
Immunohistochemistry-Paraffin: COX-2 Antibody [NBP1-85499]
Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.![COX-2 Antibody - BSA Free Immunohistochemistry-Paraffin: COX-2 Antibody [NBP1-85499]](https://resources.rndsystems.com/images/products/nbp1-85499_-immunohistochemistry-paraffin-639173124727101026.jpg "Immunohistochemistry-Paraffin: COX-2 Antibody [NBP1-85499]")
Immunohistochemistry-Paraffin: COX-2 Antibody [NBP1-85499]
Staining of human kidney shows no positivity in cells in tubules and cells in glomeruli as expected.![COX-2 Antibody - BSA Free Immunohistochemistry-Paraffin: COX-2 Antibody [NBP1-85499]](https://resources.rndsystems.com/images/products/nbp1-85499_-immunohistochemistry-paraffin-639173125288390849.jpg "Immunohistochemistry-Paraffin: COX-2 Antibody [NBP1-85499]")
Immunohistochemistry-Paraffin: COX-2 Antibody [NBP1-85499]
Staining of human seminal vesicle shows strong cytoplasmic positivity in glandular cells.Applications for COX-2 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence
Immunohistochemistry-Paraffin
Formulation, Preparation, and Storage
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Background: COX-2
Long Name
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Additional COX-2 Products
Product Documents for COX-2 Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for COX-2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for COX-2 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for COX-2 Antibody - BSA Free
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Q: I am a student researching COX-2 expression in felines and am looking for a suitable primary antibody to use in IHC. To your knowledge, have any of your COX-2 antibodies been used successfully with feline COX-2? If yes, I would be very interested in learning more. If not, would it be possible to run a sequence homology of feline COX-2 (GenBank ABM65700.1, accession EF036473.1) against your available COX-2 antibodies to determine the overlap and likelihood of cross-reactivity?
A:
We have not tested any of these antibodies for their reactivity in felines. However, we do have a number of antibodies that look like they may cross-react with feline. NBP1-85499 recognizes a region that is about 90-91% similar to feline COX2. NBP2-04015 recognizes a region in humans that is 94% similar to feline COX2. NB100-868 recognizes a region in humans at the far C-terminal 12 amino acids that is 100% identical to feline COX2. NBP1-19354 recognizes a region that is also about 95% similar to feline COX2. Only NBP1-85499 and NBP1-19354 have been tested for use in IHC. If you would like to test this antibody in an untested species and/or application and share your results with us, then I can recommend our Innovators Reward Program.