COX4 Antibody (854) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43540

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Predicted:

Chimpanzee (100%), Rhesus Macaque (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 854

Format

Azide and BSA Free
View all COX4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human COX4. The exact sequence is proprietary.

Reactivity Notes

Bovine (81%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for COX4 Antibody (854) - Azide and BSA Free

Western Blot: COX4 Antibody (854) [NBP2-43540]

Western Blot: COX4 Antibody (854) [NBP2-43540]

Western Blot: COX4 Antibody (854) [NBP2-43540] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (854)diluted at 1:1000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blot: COX4 Antibody (854) [NBP2-43540]

Western Blot: COX4 Antibody (854) [NBP2-43540]

Western Blot: COX4 Antibody (854) [NBP2-43540] - Analysis of A. 50 ug mouse heart lysate/extract 12 % SDS-PAGE COX4 antibody dilution: 1:1000
Western Blot: COX4 Antibody (854) [NBP2-43540]

Western Blot: COX4 Antibody (854) [NBP2-43540]

Western Blot: COX4 Antibody (854) [NBP2-43540] - Analysis of A. 50 ug rat muscle lysate/extract 15 % SDS-PAGE COX4 antibody [854] dilution: 1:1000.
Western Blot: COX4 Antibody (854) [NBP2-43540]

Western Blot: COX4 Antibody (854) [NBP2-43540]

Western Blot: COX4 Antibody (854) [NBP2-43540] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [854]. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blot: COX4 Antibody (854) [NBP2-43540]

Western Blot: COX4 Antibody (854) [NBP2-43540]

Western Blot: COX4 Antibody (854) [NBP2-43540] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (854) diluted at 1:1000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: COX4 Antibody (854) [NBP2-43540]

Immunohistochemistry-Paraffin: COX4 Antibody (854) [NBP2-43540]

Immunohistochemistry-Paraffin: COX4 Antibody (854) [NBP2-43540] - Human ovarian cancer. COX4 stained by COX4 antibody [854] diluted at 1:200.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min.
COX4 Antibody (854)

Immunocytochemistry/ Immunofluorescence: COX4 Antibody (854) [NBP2-43540] -

Immunocytochemistry/ Immunofluorescence: COX4 Antibody (854) [NBP2-43540] - COX4 antibody detects COX4 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: COX4 stained by COX4 antibody (NBP2-43540) diluted at 1:1000.
COX4 Antibody (854) - Azide and BSA Free

Immunohistochemistry-Paraffin: COX4 Antibody (854) [NBP2-43540] -

COX4 antibody [GT854] detects COX4 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
COX4 stained by COX4 antibody [GT854] (NBP2-43540) diluted at 1:200.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Applications for COX4 Antibody (854) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Simple Western

6 ug/ml

Western Blot

1:500-1:3000
Application Notes

See Simple Western Antibody Database for Simple Western validation: Tested in Titrated to saturation using various models, separated by Size, antibody dilution of 6 ug/mL

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: COX4

COX IV (cytochrome c oxidase subunit IV isoform 1) plays a pivotal role in respiratory function and assembly of the enzyme complex. This protein is known to have interactions with MT-CO2, MCL1, TMBIM4, MT-CO1 and TH1L. COX IV has been studied in relation to anemia, Huntington's disease, Alzheimer's disease, neuronitis, pancreatitis and several other diseases and disorders.

Long Name

Cytochrome c Oxidase Subunit IV

Alternate Names

COX4I2

Gene Symbol

COX4I1

Additional COX4 Products

Product Documents for COX4 Antibody (854) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for COX4 Antibody (854) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for COX4 Antibody (854) - Azide and BSA Free

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Protocols

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FAQs

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