CPS1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86019

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Canine

Cited:

Canine

Predicted:

Mouse (98%), Rat (97%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FKIPQKGILIGIQQSFRPRFLGVAEQLHNEGFKLFATEATSDWLNANNVPANPVAWPSQEGQNPSLSSIRKLIRDGSIDLVINLPNNNTKFVHDNYVIRRTAVDSGIPLLTNFQVTKLFAEAVQKSRKVDSKSLFHYRQYSA

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 24945279).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CPS1 Antibody - BSA Free

Immunohistochemistry-Paraffin: CPS1 Antibody [NBP1-86019]

Immunohistochemistry-Paraffin: CPS1 Antibody [NBP1-86019]

Immunohistochemistry-Paraffin: CPS1 Antibody [NBP1-86019] - Analysis in human liver and kidney tissues using NBP1-86019 antibody. Corresponding CPS1 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: CPS1 Antibody [NBP1-86019]

Immunocytochemistry/ Immunofluorescence: CPS1 Antibody [NBP1-86019]

Immunocytochemistry/Immunofluorescence: CPS1 Antibody [NBP1-86019] - Staining of human cell line A-431 shows localization to nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CPS1 Antibody [NBP1-86019]

Immunohistochemistry-Paraffin: CPS1 Antibody [NBP1-86019]

Immunohistochemistry-Paraffin: CPS1 Antibody [NBP1-86019] - Staining of human kidney shows no positivity in cells in tubules as expected.
Immunohistochemistry-Paraffin: CPS1 Antibody [NBP1-86019]

Immunohistochemistry-Paraffin: CPS1 Antibody [NBP1-86019]

Immunohistochemistry-Paraffin: CPS1 Antibody [NBP1-86019] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: CPS1 Antibody [NBP1-86019]

Immunohistochemistry-Paraffin: CPS1 Antibody [NBP1-86019]

Immunohistochemistry-Paraffin: CPS1 Antibody [NBP1-86019] - Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: CPS1 Antibody [NBP1-86019]

Immunohistochemistry-Paraffin: CPS1 Antibody [NBP1-86019]

Immunohistochemistry-Paraffin: CPS1 Antibody [NBP1-86019] - Staining of human placenta shows no positivity in trophoblastic cells as expected.
CPS1 Antibody - BSA Free Western Blot: CPS1 Antibody - BSA Free [NBP1-86019]

Western Blot: CPS1 Antibody - BSA Free [NBP1-86019]

Analysis in human liver tissue.

Applications for CPS1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CPS1

The protein encoded by the CPS1 gene is an enzyme that catalyzes the first committed step of the hepatic urea cycle, which is important in the removal of excess urea from cells. There are two isozymes of this enzyme, and the encoded protein is the mitochondrial form. Three transcript variants encoding different isoforms have been found for this gene. The shortest isoform may not be localized to the mitochondrion.

Alternate Names

carbamoyl-phosphate synthase [ammonia], mitochondrial, carbamoyl-phosphate synthase 1, mitochondrial, carbamoyl-phosphate synthetase 1, mitochondrial, carbamoylphosphate synthetase I, Carbamoyl-phosphate synthetase I, CPSase I, CPSASE1, EC 6.3.4.16

Gene Symbol

CPS1

Additional CPS1 Products

Product Documents for CPS1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CPS1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CPS1 Antibody - BSA Free

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Protocols

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