CRABP2 Antibody (004) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-89836

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 004 expressed in HEK293

Format

Azide and BSA Free
View all CRABP2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human CRABP2 (Accession#: NP_001869.1; Pro2-Glu138).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CRABP2 Antibody (004) - Azide and BSA Free

Western Blot: CRABP2 Antibody (004) [NBP2-89836]

Western Blot: CRABP2 Antibody (004) [NBP2-89836]

Western Blot: CRABP2 Antibody (004) [NBP2-89836] - Lane A: 293T Whole Cell Lysate Lane B: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:16 kDa Observed band size:14 kDa
Flow Cytometry: CRABP2 Antibody (004) [NBP2-89836]

Flow Cytometry: CRABP2 Antibody (004) [NBP2-89836]

Flow Cytometry: CRABP2 Antibody (004) [NBP2-89836] - Analysis of Human CRABP2 expression on MCF-7 cells. The cells were treated, stained with purified anti-Human CRABP2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunoprecipitation: CRABP2 Antibody (004) [NBP2-89836]

Immunoprecipitation: CRABP2 Antibody (004) [NBP2-89836]

Immunoprecipitation: CRABP2 Antibody (004) [NBP2-89836] - Lane A:0.5 mg 293T Whole Cell Lysate Lane B:0.5 mg Raji Whole Cell Lysate0.5 uL anti-CRABP2 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-CRABP2 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 16 kDa Observed band size: 16 kDa
CRABP2 Antibody (004) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: CRABP2 Antibody (004) [NBP2-89836] -

Immunocytochemistry/Immunofluorescence: CRABP2 Antibody (004) [NBP2-89836] - Staining of Human CRABP2 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89836 antibody (1:300) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Applications for CRABP2 Antibody (004) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100-1:500

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunoprecipitation

0.1-0.5 ul/mg of lysate

Western Blot

1:500-1:1000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CRABP2

Cellular retinoic acid binding protein 2 (CRABP2) is a coactivator present in the retinoic acid (RA)-dependent nuclear complex. CRABP2 has been shown to mediate retinoic acid activity in skin to influence skin cell growth and differentiation. It has also been shown to mediate the growth suppressive effects of retinoic acid in breast cancer. CRABP2's role in cell differentiation may be linked to control of the cell-cycle since it has been identified as an interacting partner of cyclin D3 in CRABPII and RAR ternary complex. Alternate names for CRABP2 include cellular retinoic acid-binding protein II, CRABP-II, retinoic acid-binding protein II, and RBP6.

Long Name

Cellular Retinoic Acid Binding Protein 2

Alternate Names

CRABP-II, RBP6

Gene Symbol

CRABP2

Additional CRABP2 Products

Product Documents for CRABP2 Antibody (004) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CRABP2 Antibody (004) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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