CRABP2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85465

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (95%), Rat (95%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CRABP2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IKQEGDTFYIKTSTTVRTTEINFKVGEEFEEQTVDGRPCKSLVKWESENKMVCEQKLLKGEGPKTSWTRELTNDGELILTMTADDVVCTRVYVRE

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 26634247).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CRABP2 Antibody - BSA Free

Western Blot: CRABP2 Antibody [NBP1-85465]

Western Blot: CRABP2 Antibody [NBP1-85465]

Western Blot: CRABP2 Antibody [NBP1-85465] - Analysis in control (vector only transfected HEK293T lysate) and CRABP2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: CRABP2 Antibody [NBP1-85465]

Immunocytochemistry/ Immunofluorescence: CRABP2 Antibody [NBP1-85465]

Immunocytochemistry/Immunofluorescence: CRABP2 Antibody [NBP1-85465] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: CRABP2 Antibody [NBP1-85465]

Immunohistochemistry-Paraffin: CRABP2 Antibody [NBP1-85465]

Immunohistochemistry-Paraffin: CRABP2 Antibody [NBP1-85465] - Staining of human vulva/anal skin shows strong nuclear and cytoplasmic positivity in squamous epithelial cells.
Western Blot: CRABP2 Antibody [NBP1-85465]

Western Blot: CRABP2 Antibody [NBP1-85465]

Western Blot: CRABP2 Antibody [NBP1-85465] - Analysis in human cell lines MCF-7 and A-549. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.

Applications for CRABP2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC reported in scientific literature (PMID: 26634247). For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CRABP2

Cellular retinoic acid binding protein 2 (CRABP2) is a coactivator present in the retinoic acid (RA)-dependent nuclear complex. CRABP2 has been shown to mediate retinoic acid activity in skin to influence skin cell growth and differentiation. It has also been shown to mediate the growth suppressive effects of retinoic acid in breast cancer. CRABP2's role in cell differentiation may be linked to control of the cell-cycle since it has been identified as an interacting partner of cyclin D3 in CRABPII and RAR ternary complex. Alternate names for CRABP2 include cellular retinoic acid-binding protein II, CRABP-II, retinoic acid-binding protein II, and RBP6.

Long Name

Cellular Retinoic Acid Binding Protein 2

Alternate Names

CRABP-II, RBP6

Entrez Gene IDs

1382 (Human)

Gene Symbol

CRABP2

UniProt

P29373

Additional CRABP2 Products

Product Documents for CRABP2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CRABP2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CRABP2 Antibody - BSA Free

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Protocols

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