CRAT Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86614

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (91%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CRAT Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids:YQILSSCRVPGPKQDTVSNFSKTKKPPTHITVVHNYQFFELDVYHSDGTPLTADQIFVQLEKIWNSSLQTNKEPVGILTSNHRNSWAKAYNTLIK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CRAT Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CRAT Antibody [NBP1-86614]

Immunocytochemistry/ Immunofluorescence: CRAT Antibody [NBP1-86614]

Immunocytochemistry/Immunofluorescence: CRAT Antibody [NBP1-86614] - Staining of human cell line A-431 shows localization to nuclear membrane. Antibody staining is shown in green.
CRAT Antibody - BSA Free Western Blot: CRAT Antibody - BSA Free [NBP1-86614]

Western Blot: CRAT Antibody - BSA Free [NBP1-86614]

Analysis using Anti-CRAT antibody NBP1-86614 (A) shows similar pattern to independent antibody NBP1-86616 (B).

Applications for CRAT Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CRAT

Carnitine acetyltransferase (CRAT) is a key enzyme in the metabolic pathway in mitochondria, peroxisomes and endoplasmic reticulum. CRAT catalyzes the reversible transfer of acyl groups from an acyl-CoA thioester to carnitine and regulates the ratio of acylCoA/CoA in the subcellular compartments. Different subcellular localizations of the CRAT mRNAs are thought to result from alternative splicing of the CRAT gene suggested by the divergent sequences in the 5' region of peroxisomal and mitochondrial CRAT cDNAs and the location of an intron where the sequences diverge. The alternatively splicing of this gene results in three distinct isoforms, one of which contains an N-terminial mitochondrial transit peptide, and has been shown to be located in mitochondria.

Alternate Names

Carnitine acetylase, Carnitine acetyltransferaseCrAT, carnitine O-acetyltransferase, CAT, CAT1EC 2.3.1.7, EC 2.3.1

Gene Symbol

CRAT

Additional CRAT Products

Product Documents for CRAT Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CRAT Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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