Cre Antibody (PSH01-93)

Novus Biologicals | Catalog # NBP3-32219

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Non-species specific

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PSH01-93 expressed in HEK293
View all Cre Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within Cre aa 1-343 / 343. (Uniprot: P06956)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cre Antibody (PSH01-93)

Cre Antibody (PSH01-93)

Western Blot: Cre Antibody (PSH01-93) [NBP3-32219]

Western Blot: Cre Antibody (PSH01-93) [NBP3-32219] - Western blot analysis of Cre on different lysates with Rabbit anti-Cre antibody (NBP3-32219) at 1/1,000 dilution.

Lane 1: 293T cell lysate
Lane 2: 293T transfected with Cre recombinase cell lysate

Lysates/proteins at 50 ng/Lane.

Predicted band size: 39 kDa
Observed band size: 39 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32219) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Cre Antibody (PSH01-93)

Immunohistochemistry: Cre Antibody (PSH01-93) [NBP3-32219]

Immunohistochemistry: Cre Antibody (PSH01-93) [NBP3-32219] - Immunofluorescence analysis of paraffin-embedded 293T transfacted with or without Cre cells labeling Cre recombinase with Rabbit anti-Cre antibody (NBP3-32219) at 1/20,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32219, green) at 1/20,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Cre Antibody (PSH01-93)

Immunohistochemistry: Cre Antibody (PSH01-93) [NBP3-32219]

Immunohistochemistry: Cre Antibody (PSH01-93) [NBP3-32219] - Immunohistochemical analysis of paraffin-embedded 293T transfacted with or without Cre cells with Rabbit anti-Cre antibody (NBP3-32219) at 1/10,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32219) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Cre Antibody (PSH01-93)

Immunocytochemistry/ Immunofluorescence: Cre Antibody (PSH01-93) [NBP3-32219]

Immunocytochemistry/ Immunofluorescence: Cre Antibody (PSH01-93) [NBP3-32219] - Immunocytochemistry analysis of 293T transfacted with or without Cre cells labeling Cre recombinase with Rabbit anti-Cre antibody (NBP3-32219) at 1/20,000 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cre antibody (NBP3-32219) at 1/20,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
Cre Antibody (PSH01-93)

Flow Cytometry: Cre Antibody (PSH01-93) [NBP3-32219]

Flow Cytometry: Cre Antibody (PSH01-93) [NBP3-32219] - Flow cytometric analysis of 293T transfacted with or without Cre cells labeling Cre recombinase.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32219, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Applications for Cre Antibody (PSH01-93)

Application
Recommended Usage

Flow Cytometry

1:1000

Immunocytochemistry/ Immunofluorescence

1:20000

Immunohistochemistry-Paraffin

1:100000

Western Blot

1:1000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cre

The Cre (cyclization recombination) enzyme, a member of a large family of recombinases, recognizes loxP which is a sequence motif of 34 bp from the PI bateriophage. If a DNA seqment is flanked by two loxP sites in the same orientation, Cre excises that seqment leaving a single loxP site in the remaining DNA molecule. The excised DNA segment is subsequently degraded. Cre and lox comprise the Cre/lox system which was first developed in the late 1980's to artificially manipulate gene expression (reviewed in Branda and Dymecki, 2004; Ghosh and Duyne, 2002; and Gilbertson, 2003). As an example, mice with the Cre protein expressing in a specific cell type are bred with mice that contain a target gene surrounded by loxP sites. When the mice are bred, the cells carrying Cre will cause those cells to lose the target gene. If the Cre gene is, for example, bound to a promoter that only allows Cre production in neuronal cells, the target gene will be deleted only in those cells. Since 1989 the Cre/lox system has been used extensively and there are numerous animal, plant and bacterial stocks that already contain the Cre gene driven by ubiquitous, tissue-specific or inducible promoters and provide a quick method for breeding experiments. The Cre/lox system has the advantage of working in almost any type of cell. This has led to its application in many experiments, for example in selectively labeling neuronal cells in the brain thereby differentiating them from other types of surrounding cells. This Cre antibody recognizes the recombinase protein, Cre. Cre is a 343 amino acid protein, GenBank no. AAQ14086.1.

Alternate Names

Cyclization recombinase

Additional Cre Products

Product Documents for Cre Antibody (PSH01-93)

Certificate of Analysis

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Product Specific Notices for Cre Antibody (PSH01-93)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Cre Antibody (PSH01-93)

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  • Q: A customer is looking for a Cre Antibody, raised in either rabbit or goat, suitable for IHC-F. Would you know of a suitable Cre antibody that fits the above criteria and also been published for IHC-Fr? If known, can you also provide details of the publication?

    A:

    With regards to your customer's inquiry on our Cre antibodies, we do have a wide range of antibodies that would be suitable to use in IHC for frozen sections. NB100-56134 for instance has been tested and validated for IHC-fry and there is one cited PubMed publication on it as well.

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