Key Product Details

Species Reactivity

Mouse, Cre/lox System

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Rabbit IgG
View all Cre Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a portion of bacteriophage protein CRE (within amino acids 50-100). [UniProt# P06956]

Reactivity Notes

Specific to Cre/lox system (Bacteriophage). Shown to detect Cre protein in Cre-transfected human cells (HeLa) and Cre-transgenic mice. Mouse reactivity reported in scientific literature (PMID: 28194015).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cre Antibody [FITC]

Immunohistochemistry: Cre Antibody [FITC] [NB100-56133F] - Perfused mouse brain tissue. Dilution is 1:4000. This image was submitted via customer Review.

Applications for Cre Antibody [FITC]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Cre

Cre recombinase or cyclization recombination enzyme is a bacteriophage-P1 enzyme belonging to large family of recombinases which are required for maintenance of the phage genome as a monomeric plasmid in the lysogenic state. Cre is well known for facilitating a site-specific recombination between two 34 base pair loxP sites. If a DNA seqment is flanked by two loxP sites in the same orientation, Cre excises that seqment leaving a single loxP site in the remaining DNA molecule, and the excised DNA segment is subsequently degraded. Cre and lox comprises Cre/lox system which has been used extensively for multiple in vitro and in vivo applications including targeted gene deletions, gene-specific humanized animal models, as well as in plants and microorganisms stocks.

Alternate Names

Cyclization recombinase

Additional Cre Products

Product Documents for Cre Antibody [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Cre Antibody [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cre Antibody [FITC]

Customer Reviews for Cre Antibody [FITC]

There are currently no reviews for this product. Be the first to review Cre Antibody [FITC] and earn rewards!

Have you used Cre Antibody [FITC]?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Cre Antibody [FITC]

Showing  1 - 1 of 1 FAQ Showing All

  • Q: A customer is looking for a Cre Antibody, raised in either rabbit or goat, suitable for IHC-F. Would you know of a suitable Cre antibody that fits the above criteria and also been published for IHC-Fr? If known, can you also provide details of the publication?

    A: With regards to your customer's inquiry on our Cre antibodies, we do have a wide range of antibodies that would be suitable to use in IHC for frozen sections. NB100-56134 for instance has been tested and validated for IHC-fry and there is one cited PubMed publication on it as well.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies