Key Product Details

Species Reactivity

Validated:

Mouse, Cre/lox System

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cre Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a portion of bacteriophage protein CRE (within amino acids 50-100). [UniProt# P06956]

Reactivity Notes

Specific to Cre/lox system (Bacteriophage). Shown to detect Cre protein in Cre-transfected human cells (HeLa) and Cre-transgenic mice. Mouse reactivity reported in scientific literature (PMID: 28194015).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cre Antibody - BSA Free

Western Blot: Cre AntibodyBSA Free [NB100-56133]

Western Blot: Cre AntibodyBSA Free [NB100-56133]

Western Blot: Cre Antibody [NB100-56133] - Analysis of Cre using NB100-56133 at 1:2000. Lane 1: HeLa cells stably transfected with a CRE expression plasmid. Lane 2: HeLa cells transfected with a neo plasmid (negative control).
Immunohistochemistry: Cre Antibody - BSA Free [NB100-56133]

Immunohistochemistry: Cre Antibody - BSA Free [NB100-56133]

Cre-Antibody---BSA-Free-Immunohistochemistry-NB100-56133-img0008.jpg
Immunohistochemistry: Cre Antibody - BSA Free [NB100-56133]

Immunohistochemistry: Cre Antibody - BSA Free [NB100-56133]

Immunohistochemistry: Cre Antibody [NB100-56133] - FFPE tissue section from the cerebellum of a Cre transgenic mouse using NB100-56133 at 1:2000. In this mouse line, Cre is expressed in the brain, predominantly in the nuclei of most types of neurons. The arrow denotes an example of a stained neuron. Cre staining is also seen in the cytosol of cerebellar Purkinje cells. Hematoxylin-eosin counterstain.
Immunohistochemistry: Cre Antibody - BSA Free [NB100-56133]

Immunohistochemistry: Cre Antibody - BSA Free [NB100-56133]

Immunohistochemistry: Cre Antibody [NB100-56133] - FFPE tissue section from the cerebellum of a Cre transgenic mouse using NB100-56133 at 1:2000. In this mouse line, Cre is expressed in the neurons brain. This mouse line also contains a LacZ reporter gene that becomes transcriptionally active upon Cre-mediated recombination. Brain tissue slices were stained with X-gal (blue color) prior to embedding. Cre staining is brown. Nuclear Red counterstain.
Immunohistochemistry: Cre Antibody - BSA Free [NB100-56133]

Immunohistochemistry: Cre Antibody - BSA Free [NB100-56133]

Immunohistochemistry: Cre Antibody [NB100-56133] - Enkephalin expression in the Paraventricular nucleus of the hypothalamus (PVN) in mouse brain. Antibody at 1:500. Image from verified customer review.

Applications for Cre Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:500

Immunohistochemistry-Frozen

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

1:50 - 1:200

Western Blot

1:1000 - 1:4000

Reviewed Applications

Read 1 review rated 5 using NB100-56133 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cre

Cre recombinase or cyclization recombination enzyme is a bacteriophage-P1 enzyme belonging to large family of recombinases which are required for maintenance of the phage genome as a monomeric plasmid in the lysogenic state. Cre is well known for facilitating a site-specific recombination between two 34 base pair loxP sites. If a DNA seqment is flanked by two loxP sites in the same orientation, Cre excises that seqment leaving a single loxP site in the remaining DNA molecule, and the excised DNA segment is subsequently degraded. Cre and lox comprises Cre/lox system which has been used extensively for multiple in vitro and in vivo applications including targeted gene deletions, gene-specific humanized animal models, as well as in plants and microorganisms stocks.

Alternate Names

Cyclization recombinase

Additional Cre Products

Product Documents for Cre Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Cre Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cre Antibody - BSA Free

Customer Reviews for Cre Antibody - BSA Free (1)

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Customer Images

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  • Cre Antibody
    Name: Hayk Simonyan
    Application: Immunohistochemistry
    Sample Tested: Adult mouse brain tissue
    Species: Mouse
    Verified Customer | Posted 05/24/2017
    IHC shows Enkephalin expression in the Paraventricular nucleus of the hypothalamus (PVN) in mouse brain. Dilution 1:500
    Cre Antibody - BSA Free NB100-56133

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Protocols

View specific protocols for Cre Antibody - BSA Free (NB100-56133):


Western Blot Protocol
1. Perform SDS-PAGE (4-12% MOPS) on samples to be analyzed, loading 35 ug of total protein per lane.
2. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
3. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
4. Rinse the blot in TBS for approximately 5 minutes.
5. Block the membrane using 5% NFDM + 1% BSA in TBS + Tween, 1 hour at RT.
6. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each.
7. Dilute the rabbit anti-cre primary antibody (NB100-56133) in blocking buffer and incubate 1 hour at room temperature.
8. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each.
9. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).
Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%, provided it does not interfere with antibody-antigen binding.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Cre Antibody - BSA Free

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  • Q: A customer is looking for a Cre Antibody, raised in either rabbit or goat, suitable for IHC-F. Would you know of a suitable Cre antibody that fits the above criteria and also been published for IHC-Fr? If known, can you also provide details of the publication?

    A:

    With regards to your customer's inquiry on our Cre antibodies, we do have a wide range of antibodies that would be suitable to use in IHC for frozen sections. NB100-56134 for instance has been tested and validated for IHC-fry and there is one cited PubMed publication on it as well.

  • Q: Can I use this antibody for protein immunoblots of flox/flox and flox/flox cre animals to show cre is expressed only in the flox/flox cre animals?

    A: Our NB100-56133 Cre antibody is specific to the cre enzyme. When using this antibody, you should only detect a band on your blot when the cre enzyme is present. There should be no detection of your floxed mice as long as cre is not expressed or added.

  • Q: A customer is looking for a Cre Antibody, raised in either rabbit or goat, suitable for IHC-F. Would you know of a suitable Cre antibody that fits the above criteria and also been published for IHC-Fr? If known, can you also provide details of the publication?

    A:

    With regards to your customer's inquiry on our Cre antibodies, we do have a wide range of antibodies that would be suitable to use in IHC for frozen sections. NB100-56134 for instance has been tested and validated for IHC-fry and there is one cited PubMed publication on it as well.

  • Q: Can I use this antibody for protein immunoblots of flox/flox and flox/flox cre animals to show cre is expressed only in the flox/flox cre animals?

    A: Our NB100-56133 Cre antibody is specific to the cre enzyme. When using this antibody, you should only detect a band on your blot when the cre enzyme is present. There should be no detection of your floxed mice as long as cre is not expressed or added.

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