CREB [p Ser133] Antibody - Azide Free

Western Blot: CREB [p Ser133] Antibody [NB300-273]

Western Blot: CREB [p Ser133] Antibody [NB300-273] - Rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~45 kDa CREB phosphorylated at Ser133 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase lambda-Ptase, 800 units/1mg protein for 30 min).

Immunohistochemistry-Frozen: CREB [p Ser133] Antibody [NB300-273]

Immunohistochemistry-Frozen: CREB [p Ser133] Antibody [NB300-273] - IHC Analysis of pCREB S133 (red) in frozen sections from mouse piriform cortex. Cell nuclei are visualized with DAPI DNA stain (blue). NB300-273 was used at a dilution of 1:1000 and incubated for 48 hours at 4C. No antigen retrieval step was performed.

Western Blot: CREB [p Ser133] Antibody - Azide Free [NB300-273] -

C. difficile toxins increases the levels of phosphorylated CREB, which are involved in EGCs death induced by these toxins in vitro. (A) Representative Western Blot images showing phosphorylated CREB and tubulin (loading control) protein expression. (B) levels of pCREB (mean +/- s.e.m.) measured by ELISA in EGCs challenged with TcdA and TcdB for 4h. (C) Cell death analyzed by RealTime-Glo annexin V apoptosis assay (mean +/- s.e.m, n=6) in enteroglial cell (EGC/PK060399) challenged with TcdA and TcdB for 18 h in the presence and/or absence of 0.5 uM 666-15 (a CREB inhibitor) and 300 uM ATL313 (an A2a-selective adenosine receptor agonist), added one hour prior to C. difficile toxin challenge. (B, C) One-way ANOVA followed by Turkey test was used. These experiments are from two different replicates. ***p<0.0001, **p<0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36726986), licensed under a CC-BY license. Not internally tested by Novus Biologicals.