CREB Antibody

Chromatin Immunoprecipitation: CREB Antibody [NBP2-22516] - Analysis performed using cross-linked chromatin from colon carcinoma cells treated with serum. IP was performed using a multiplex microplate Matrix ChIP assay of a CREB polyclonal antibody. Chromatin aliquots from cells were used per ChIP pull-down. Quantitative PCR data one in quadruplicate using 1ul of eluted DNA in 2ul SYBR real-time PCR reactions containing primers to amplify -15kb upstream of Egr1 or exon-1 or exon-2 of Egr1.Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM. A schematic representation of the Egr-1 locus is shown; xes represent exons (black boxes = translated regions, white boxes = untranslated regions), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by Egr-1 primers are represented by black bars. Data courtesy of the Innovators Program.

Immunocytochemistry/ Immunofluorescence: CREB Antibody [NBP2-22516]

Immunocytochemistry/Immunofluorescence: CREB Antibody [NBP2-22516] - Analysis of CREB (green) showing staining in the nucleus of SK-N-MC cells (right) compared to a negative control without primary antibody (left).

Immunohistochemistry-Paraffin: CREB Antibody [NBP2-22516]

Immunohistochemistry-Paraffin: CREB Antibody [NBP2-22516] - Analysis showing staining in the nucleus of mouse brain tissue (right) compared to a negative control without primary antibody (left).

Immunohistochemistry-Paraffin: CREB Antibody [NBP2-22516]

Immunohistochemistry-Paraffin: CREB Antibody [NBP2-22516] - Analysis showing staining in the nucleus of human lung adenocarcinoma (right) compared to a negative control without primary antibody (left).