CREM Antibody (3B5) - Azide and BSA Free

Novus Biologicals | Catalog # H00001390-M02

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3B5

Format

Azide and BSA Free
View all CREM Primary Antibodies »

Product Specifications

Immunogen

CREM (NP_853549, 201 a.a. ~ 300 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ATGDMPTYQIRAPTAALPQGVVMAASPGSLHSPQQLAEEATRKRELRLMKNREAAKECRRRKKEYVKCLESRVAVLEVQNKKLIEELETLKDICSPKTDY

Specificity

CREM (3B5)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CREM Antibody (3B5) - Azide and BSA Free

Western Blot: CREM Antibody (3B5) [H00001390-M02]

Western Blot: CREM Antibody (3B5) [H00001390-M02]

Western Blot: CREM Antibody (3B5) [H00001390-M02] - CREM monoclonal antibody (M02), clone 3B5. Analysis of CREM expression in human liver.
Immunocytochemistry/ Immunofluorescence: CREM Antibody (3B5) [H00001390-M02]

Immunocytochemistry/ Immunofluorescence: CREM Antibody (3B5) [H00001390-M02]

Immunocytochemistry/Immunofluorescence: CREM Antibody (3B5) [H00001390-M02] - UTGB1, primary glioblastoma cell line, expressing the EWSR1-CREM fusion, stained using CREM antibody at 1:200. Secondary Antibody: Alexa Fluor 568 conjugate. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: CREM Antibody (3B5) [H00001390-M02]

Immunocytochemistry/ Immunofluorescence: CREM Antibody (3B5) [H00001390-M02]

Immunocytochemistry/Immunofluorescence: CREM Antibody (3B5) [H00001390-M02] - Analysis of monoclonal antibody to CREM on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: CREM Antibody (3B5) [H00001390-M02] - Detection limit for recombinant GST tagged CREM is 0.03 ng/ml as a capture antibody.
Knockdown Validated: CREM Antibody (3B5) [H00001390-M02]

Western Blot: CREM Antibody (3B5) [H00001390-M02]

Western Blot: CREM Antibody (3B5) [H00001390-M02] - UTGB1, primary glioblastoma cell line, expressing the EWSR1-CREM fusion, treated with non-target siRNA and different concentrations of CREM siRNA. Image from verified customer review.

Applications for CREM Antibody (3B5) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Reviewed Applications

Read 2 reviews rated 4.5 using H00001390-M02 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CREM

This gene encodes a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. It is an important component of cAMP-mediated signal transduction during the spermatogenetic cycle, as well as other complex processes. Alternative promoter and translation initiation site usage allows this gene to exert spatial and temporal specificity to cAMP responsiveness. Multiple alternatively spliced transcript variants encoding several different isoforms have been found for this gene, with some of them functioning as activators and some as repressors of transcription.

Alternate Names

cAMP response element modulator, cAMP responsive element modulator, cAMP-responsive element modulator, hCREM 2alpha-b protein, hCREM 2beta-a protein, hCREM-2, ICER, Inducible cAMP early repressor, inducible cAMP early repressor ICER, MGC111110, MGC17881, MGC41893

Entrez Gene IDs

1390 (Human)

Gene Symbol

CREM

OMIM

123812 (Human)

UniProt

NP_853549 (Human)

Additional CREM Products

Product Documents for CREM Antibody (3B5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CREM Antibody (3B5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CREM Antibody (3B5) - Azide and BSA Free

Customer Reviews for CREM Antibody (3B5) - Azide and BSA Free (2)

4.5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
50%
3 Stars
0%
2 Stars
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1 Stars
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • CREM Antibody (3B5)
    Name: Vanina D. Heuser
    Application: Immunocytochemistry
    Sample Tested: Primary Glioblastoma cell line UTGB1 and human glioblastoma
    Species: Human
    Verified Customer | Posted 08/30/2018
    UTGB1 primary glioblastoma cell line expressing the EWSR1-CREM fusion stained using 1:200 mouse x CREM antibody. Secondary ANtibody: Alexa 568 x Mouse.
    CREM Antibody (3B5) - Azide and BSA Free H00001390-M02
  • CREM Antibody (3B5)
    Name: Vanina D. Heuser
    Application: Western Blot
    Sample Tested: Primary Glioblastoma cell line UTGB1
    Species: Human
    Verified Customer | Posted 08/30/2018
    UTGB1 primary glioblastoma cell line expression the EWSR1-CREM fusion treated with non target siRNA and different concentrations of CREM siRNA.
    CREM Antibody (3B5) - Azide and BSA Free H00001390-M02

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Protocols

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FAQs

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