Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Canine, Chicken, Mammal

Cited:

Mouse, Rat, Mammal

Predicted:

Primate (94%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CRHR2 Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 75-125 of human CRHR2 was used as the immunogen.

Reactivity Notes

Mammal reactivity reported in scientific literature (PMID:33344730) 94% sequence identity with Rhesus monkey

Specificity

There are three isoforms of CRHR2 in human: alpha is 411 amino acids, beta is 438 amino acids, and gamma is 397 amino acids.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CRHR2/CRF2 Antibody - BSA Free

Western Blot: CRHR2/CRF2 Antibody [NB100-56485]

Western Blot: CRHR2/CRF2 Antibody [NB100-56485]

Western Blot: CRHR2/CRF2 Antibody [NB100-56485] - Analysis of CRHR2 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Both of the isoforms detected by this antibody.
Simple Western: CRHR2/CRF2 Antibody [NB100-56485]

Simple Western: CRHR2/CRF2 Antibody [NB100-56485]

Simple Western: CRHR2/CRF2 Antibody [NB100-56485] - Shows a specific band for Corticotropin Releasing Factor 2 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
CRHR2/CRF2 Antibody - BSA Free

Western Blot: CRHR2/CRF2 Antibody - BSA Free [NB100-56485] -

Hypothalamic neuronal activity under 5-HT1Areceptor agonist treatment. After fluoxetine injection for 4 weeks, hypothalamic CRH neuronal activity (CRH, CRH_R2 and c-Fos) before and after challenge with 8-OH-DPAT was evaluated by immunofluorescent staining (A) and western blotting (B) analysis. In the hypothalamic plasma membrane, the protein expression levels of the 5-HT1A receptors, GIRK1 and GIRK2 were measured (C). The protein expression levels of BDNF, phosphorylated ERK1/2 and CREB in whole hypothalamic lysate were analyzed (D). The data are expressed as the mean +/- SD (n = 4/group) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38191373), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
CRHR2/CRF2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CRHR2/CRF2 Antibody - BSA Free [NB100-56485] -

Hypothalamic neuronal activity under 5-HT1Areceptor agonist treatment. After fluoxetine injection for 4 weeks, hypothalamic CRH neuronal activity (CRH, CRH_R2 and c-Fos) before and after challenge with 8-OH-DPAT was evaluated by immunofluorescent staining (A) and western blotting (B) analysis. In the hypothalamic plasma membrane, the protein expression levels of the 5-HT1A receptors, GIRK1 and GIRK2 were measured (C). The protein expression levels of BDNF, phosphorylated ERK1/2 and CREB in whole hypothalamic lysate were analyzed (D). The data are expressed as the mean +/- SD (n = 4/group) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38191373), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for CRHR2/CRF2 Antibody - BSA Free

Application
Recommended Usage

Simple Western

1:200

Western Blot

0.5-2 ug/ml
Application Notes
See Simple Western Antibody Database for Simple Western validation: Tested in Human Brain lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 49 kDa

Reviewed Applications

Read 1 review rated 5 using NB100-56485 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CRHR2

CRHR2 is a novel member of Secretin-family-GPCR proteins that functions as a functional receptor for CRF. It is expressed as 3 isoforms; alpha, beta and gamma. These isoforms primarily differ in their N-terminal sequence and tissue distribution. Apart from CRF, other ligands of CRHR2 include UcnI, UcnII and UcnIII. CRHR2 is involved in stress responses, cardiovascular function and gastric motility. Studies on CRHR2 deficient mice suggested a central anxiolytic effect of CRHR2. Northern Blot analysis detects CRHR2 expression in brain, hypothalamus, heart, GI, lung, skeletal muscle and vasculature.

Long Name

Corticotropin Releasing Hormone Receptor 2

Alternate Names

CRF-RB, CRF2, HM-CRF

Entrez Gene IDs

1395 (Human); 12922 (Mouse); 64680 (Rat)

Gene Symbol

CRHR2

UniProt

NP_001874 (Human)

Additional CRHR2 Products

Product Documents for CRHR2/CRF2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CRHR2/CRF2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CRHR2/CRF2 Antibody - BSA Free

Customer Reviews for CRHR2/CRF2 Antibody - BSA Free (1)

5 out of 5
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Customer Images

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: brain lysate
    Species: Other
    Verified Customer | Posted 09/11/2014
    analysis of CRFR2 in prairie vole brain lysate
    CRHR2/CRF2 Antibody - BSA Free NB100-56485

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