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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence YGRNCEHDVRKEN corresponding to internal region according to NP_003203.1.
Epitope
YGRNCEHDVRKEN
Marker
Embryonic Stem Cell Marker
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Novus Biologicals Goat CRIPTO Antibody (NBP1-68841) is a polyclonal antibody validated for use in IHC and ELISA. Anti-CRIPTO Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for CRIPTO Antibody
Immunohistochemistry-Paraffin: CRIPTO Antibody [NBP1-68841]
Immunohistochemistry-Paraffin: CRIPTO Antibody [NBP1-68841] - Cripto1 Antibody [NBP1-68841] (3.8ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Applications for CRIPTO Antibody
Application
Recommended Usage
Immunohistochemistry
3 - 5 ug/ml
Immunohistochemistry-Paraffin
3 - 5 ug/ml
Peptide ELISA
Detection limit 1:128000
Application Notes
IHC-P: Human placenta shows stainng assocated with nuclei in some trophoblasts and weak cytoplasm staining in cytotrophoblasts.
Reviewed Applications
Read 1 review rated 5 using NBP1-68841 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Cripto
Long Name
Teratocarcinoma-derived Growth Factor/Cripto Growth Factor
Alternate Names
CFC-2, TDGF-1, TDGF1
Gene Symbol
TDGF1
UniProt
Additional Cripto Products
Product Documents for CRIPTO Antibody
Product Specific Notices for CRIPTO Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for CRIPTO Antibody
Customer Reviews for CRIPTO Antibody (1)
5 out of 5
1 Customer Rating
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Customer Images
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Application: ImmunohistochemistrySample Tested: 8Species: MouseVerified Customer | Posted 06/09/2017IHCtraditional protocol without unmasking steps (as recommended for human samples)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for CRIPTO Antibody
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Q: Our customer want to know the positive control of: NB100-91794 and NBP1-68841. Could you let me know about it?
A: In regards to NB100-91794, positive controls would be Cos7 cells for WB or Human breast carcinoma tissue for IHC. For NBP1-68841, the positive control is human placenta for IHC.
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