CRMP2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31762

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CRMP2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human CRMP2. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (87%), Xenopus tropicalis (88%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CRMP2 Antibody - BSA Free

Western Blot: CRMP2 Antibody [NBP1-31762]

Western Blot: CRMP2 Antibody [NBP1-31762]

Western Blot: CRMP2 Antibody [NBP1-31762] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CRMP2 antibody [N1N3] diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: CRMP2 Antibody [NBP1-31762]

Immunohistochemistry-Paraffin: CRMP2 Antibody [NBP1-31762]

Immunohistochemistry-Paraffin: CRMP2 Antibody [NBP1-31762] - Rat brain. CRMP2 stained by CRMP2 antibody [N1N3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: CRMP2 Antibody [NBP1-31762]

Western Blot: CRMP2 Antibody [NBP1-31762]

Western Blot: CRMP2 Antibody [NBP1-31762] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CRMP2 antibody [N1N3] diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: CRMP2 Antibody [NBP1-31762]

Immunohistochemistry-Paraffin: CRMP2 Antibody [NBP1-31762]

Immunohistochemistry-Paraffin: CRMP2 Antibody [NBP1-31762] - DLD1 xenograft, using CRMP2 antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
CRMP2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CRMP2 Antibody [NBP1-31762] -

CRMP2 antibody [N1N3] detects CRMP2 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CRMP2 stained by CRMP2 antibody [N1N3] (NBP1-31762) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.
Blue: Fluoroshield with DAPI.

Applications for CRMP2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:5000-1:100000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CRMP2

Collapsin response mediator proteins (CRMPs) are cytosolic phosphoproteins involved in neuronal differentiation and axonal guidance. CRMP2 was previously shown to mediate the repulsive effect of Sema3A on axons and to participate in axonal specification. The CRMPs appear to play a complex role in axon growth as well as microtubule dynamics and axon induction. CRMPs localize to the lamellipodia and filopodia of axonal growth cones, suggesting a role in axon guidance. Moreover, CRMP2 is upregulated after axotomy, and appears to increase the formation of axon-type processes from hippocampal neurons. CRMP2 has been reported to bind tubulin dimers directly and modulate microtubule assembly. CRMPs have also been implicated in the pathogenesis of a paraneoplastic neurologic syndrome. Interaction studies have implicated phospholipase D2 (PLD2), the cytosolic tyrosine kinase Fes, and intersectin in CRMP function. Hyperphosphorylation of CRMP2 is an early event in the progression of Alzheimer's disease.

Alternate Names

Collapsin response mediator protein 2, CRMP-2, CRMP2N2A3, DHPRP2, dihydropyrimidinase-like 2, dihydropyrimidinase-like 2 long form, dihydropyrimidinase-related protein 2, DRP2collapsin response mediator protein hCRMP-2, DRP-2ULIP-2, ULIP2, Unc-33-like phosphoprotein 2

Gene Symbol

DPYSL2

Additional CRMP2 Products

Product Documents for CRMP2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CRMP2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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