CRMP2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85448

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CRMP2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DFFQGTKAALAGGTTMIIDHVVPEPGTSLLAAFDQWREWADSKSCCDYSLHVDISEWHKGIQEEMEALVKDHGVNSFLVYMAFKDRFQLTDCQIYEVLSVIRDIGAIAQVHAENGDIIAEEQQRILDLGITGPEGHVLSR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CRMP2 Antibody - BSA Free

Western Blot: CRMP2 Antibody [NBP1-85448]

Western Blot: CRMP2 Antibody [NBP1-85448]

Western Blot: CRMP2 Antibody [NBP1-85448] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: CRMP2 Antibody [NBP1-85448]

Immunocytochemistry/ Immunofluorescence: CRMP2 Antibody [NBP1-85448]

Immunocytochemistry/Immunofluorescence: CRMP2 Antibody [NBP1-85448] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CRMP2 Antibody [NBP1-85448]

Immunohistochemistry-Paraffin: CRMP2 Antibody [NBP1-85448]

Immunohistochemistry-Paraffin: CRMP2 Antibody [NBP1-85448] - Staining of human lung shows cytoplasmic and membranous positivity in pneumocytes.
Western Blot: CRMP2 Antibody [NBP1-85448]

Western Blot: CRMP2 Antibody [NBP1-85448]

Western Blot: CRMP2 Antibody [NBP1-85448] - Analysis in human cell line U-87 MG.

Applications for CRMP2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25 - 2 ug/mL

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04 - 0.4 ug/mL
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 2 reviews rated 3 using NBP1-85448 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CRMP2

Collapsin response mediator proteins (CRMPs) are cytosolic phosphoproteins involved in neuronal differentiation and axonal guidance. CRMP2 was previously shown to mediate the repulsive effect of Sema3A on axons and to participate in axonal specification. The CRMPs appear to play a complex role in axon growth as well as microtubule dynamics and axon induction. CRMPs localize to the lamellipodia and filopodia of axonal growth cones, suggesting a role in axon guidance. Moreover, CRMP2 is upregulated after axotomy, and appears to increase the formation of axon-type processes from hippocampal neurons. CRMP2 has been reported to bind tubulin dimers directly and modulate microtubule assembly. CRMPs have also been implicated in the pathogenesis of a paraneoplastic neurologic syndrome. Interaction studies have implicated phospholipase D2 (PLD2), the cytosolic tyrosine kinase Fes, and intersectin in CRMP function. Hyperphosphorylation of CRMP2 is an early event in the progression of Alzheimer's disease.

Alternate Names

Collapsin response mediator protein 2, CRMP-2, CRMP2N2A3, DHPRP2, dihydropyrimidinase-like 2, dihydropyrimidinase-like 2 long form, dihydropyrimidinase-related protein 2, DRP2collapsin response mediator protein hCRMP-2, DRP-2ULIP-2, ULIP2, Unc-33-like phosphoprotein 2

Gene Symbol

DPYSL2

Additional CRMP2 Products

Product Documents for CRMP2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CRMP2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for CRMP2 Antibody - BSA Free (2)

3 out of 5
2 Customer Ratings
5 Stars
0%
4 Stars
50%
3 Stars
0%
2 Stars
50%
1 Stars
0%

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  • CRMP2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: microglia
    Species: Rat
    Verified Customer | Posted 05/21/2021
    Rat primary microglia under no stimulated condition.
    CRMP2 Antibody - BSA Free NBP1-85448
  • CRMP2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MICROGLIA and Human microglia cell lysate
    Species: Rat
    Verified Customer | Posted 05/20/2021
    LYSATES OF RAT MICROGLIA (10 MICROG)
    CRMP2 Antibody - BSA Free NBP1-85448
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.

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Protocols

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