CROP Antibody - BSA Free
Novus Biologicals | Catalog # NB100-74625
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human cisplatin resistance-associated overexpressed protein using the numbering given in entry NP_057508.2 (GeneID 51747).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CROP Antibody - BSA Free
Western Blot: CROP Antibody [NB100-74625]
Western Blot: CROP Antibody [NB100-74625] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-CROP/Luc7A antibody NB100-74625 used for IP at 3 mcg/mg lysate. CROP/Luc7A was also immunoprecipitated by rabbit anti-CROP/Luc7A antibody NB100-74626, which recognizes a downstream epitope.Immunohistochemistry: CROP Antibody [NB100-74625]
Immunohistochemistry: CROP Antibody [NB100-74625] - Sample: FFPE section of human testicular seminoma (left) and mouse colon carcinoma (right). Antibody: Affinity purified rabbit anti-CROP/Luc7A used at a dilution of 1:1,000 (1ug/ml). Detection: DABImmunohistochemistry-Paraffin: CROP Antibody [NB100-74625]
Immunohistochemistry-Paraffin: CROP Antibody [NB100-74625] - FFPE section of human thyroid carcinoma. Affinity purified rabbit anti-CROP/Luc7A used at a dilution of 1:250.Applications for CROP Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:100-1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CROP
Alternate Names
cAMP regulatory element-associated protein 1, cisplatin resistance associated overexpressed protein, cisplatin resistance-associated overexpressed protein, Cisplatin resistance-associated-overexpressed protein, CREAP1, CREAP-1LUC7ACRA, CRE-associated protein 1, CROPFLJ11063, hLuc7A, Luc7A, LUC7-like 3 (S. cerevisiae), luc7-like protein 3, O48, OA48-18, Okadaic acid-inducible phosphoprotein OA48-18
Entrez Gene IDs
51747 (Human)
Gene Symbol
LUC7L3
UniProt
Additional CROP Products
Product Documents for CROP Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for CROP Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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