CROT Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85502

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CROT Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VRWGDKSYNLISFSNGVFGCNCDHAPFDAMIMVNISYYVDEKIFQNEGRWKGSEKVRDIPLPEELIFIVDEKVLNDINQAKAQYLR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CROT Antibody - BSA Free

Western Blot: CROT Antibody [NBP1-85502]

Western Blot: CROT Antibody [NBP1-85502]

Western Blot: CROT Antibody [NBP1-85502] - Analysis in control (vector only transfected HEK293T lysate) and CROT over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: CROT Antibody [NBP1-85502]

Immunohistochemistry-Paraffin: CROT Antibody [NBP1-85502]

Immunohistochemistry-Paraffin: CROT Antibody [NBP1-85502] - Staining in human thyroid gland and skeletal muscle tissues using anti-CROT antibody. Corresponding CROT RNA-seq data are presented for the same tissues.
Western Blot: CROT Antibody [NBP1-85502]

Western Blot: CROT Antibody [NBP1-85502]

Western Blot: CROT Antibody [NBP1-85502] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Western Blot: CROT Antibody [NBP1-85502]

Western Blot: CROT Antibody [NBP1-85502]

Western Blot: CROT Antibody [NBP1-85502] - Analysis using Anti-CROT antibody NBP1-85502 (A) shows similar pattern to independent antibody NBP1-85501 (B).
Immunohistochemistry-Paraffin: CROT Antibody [NBP1-85502]

Immunohistochemistry-Paraffin: CROT Antibody [NBP1-85502]

Immunohistochemistry-Paraffin: CROT Antibody [NBP1-85502] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: CROT Antibody [NBP1-85502]

Immunohistochemistry-Paraffin: CROT Antibody [NBP1-85502]

Immunohistochemistry-Paraffin: CROT Antibody [NBP1-85502] - Staining of human thyroid gland shows high expression.

Applications for CROT Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CROT

Carnitine octanoyltransferase (EC 2.3.1.137) is a carnitine acyltransferase that catalyzes the reversible transfer of fatty acyl groups between CoA and carnitine. This provides a crucial step in the transport of medium- and long-chain acyl-CoA out of the mammalian peroxisome to the cytosol and mitochondria. See also CRAT (MIM 600184). Van der Leij et al. (2000) [PubMed 11001805] reviewed the function, structural features, and phylogenetics of human carnitine acyltransferase genes, including CROT.[supplied by OMIM]

Alternate Names

carnitine O-octanoyltransferase, COTperoxisomal carnitine O-octanoyltransferase, EC 2.3.1, EC 2.3.1.137, peroxisomal carnitine acyltransferase, peroxisomal carnitine octanoyltransferase

Gene Symbol

CROT

Additional CROT Products

Product Documents for CROT Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CROT Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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