CRYBB2 Antibody (1F1) - Azide and BSA Free

Novus Biologicals | Catalog # H00001415-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1F1

Format

Azide and BSA Free
View all CRYBB2 Primary Antibodies »

Product Specifications

Immunogen

CRYBB2 (NP_000487.1, 1 a.a. ~ 205 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASDHQTQAGKPQSLNPKIIIFEQENFQGHSHELNGPCPNLKETGVEKAGSVLVQAGPWVGYEQANCKGEQFVFEKGEYPRWDSWTSSRRTDSLSSLRPIKVDSQEHKIILYENPNFTGKKMEIIDDDVPSFHAHGYQEKVSSVRVQSGTWVGYQYPGYRGLQYLLEKGDYKDSSDFGAPHPQVQSVRRIRDMQWHQRGAFHPSN

Specificity

This product is specific for Human CRYBB2 monoclonal antibody (M02), clone 1F1 [Gene ID: 1415].

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CRYBB2 Antibody (1F1) - Azide and BSA Free

Western Blot: CRYBB2 Antibody (1F1) [H00001415-M02]

Western Blot: CRYBB2 Antibody (1F1) [H00001415-M02]

Western Blot: CRYBB2 Antibody (1F1) [H00001415-M02] - Analysis of CRYBB2 expression in transfected 293T cell line by CRYBB2 monoclonal antibody (M02), clone 1F1.Lane 1: CRYBB2 transfected lysate (Predicted MW: 23.4 KDa).Lane 2: Non-transfected lysate.
Western Blot: CRYBB2 Antibody (1F1) [H00001415-M02]

Western Blot: CRYBB2 Antibody (1F1) [H00001415-M02]

Western Blot: CRYBB2 Antibody (1F1) [H00001415-M02] - analysis of CRYBB2 in stably transfected HEK293T cell lysate. Note: membrane was blocked overnight in 3% BSA in TBST at 4C to reduce the background. Image from verified customer review.
ELISA: CRYBB2 Antibody (1F1) [H00001415-M02]

ELISA: CRYBB2 Antibody (1F1) [H00001415-M02]

ELISA: CRYBB2 Antibody (1F1) [H00001415-M02] - Detection limit for recombinant GST tagged CRYBB2 is 3 ng/ml as a capture antibody.

Applications for CRYBB2 Antibody (1F1) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:100-1:2000
Application Notes
Use in ICC/IF was reported in scientific literature (PMID: 31511085).

Reviewed Applications

Read 1 review rated 4 using H00001415-M02 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CRYBB2

Alternate Names

Beta-B2 crystallin, beta-crystallin B2, Beta-crystallin Bp, CCA2, CRYB2A, CRYB2D22S665, crystallin, beta B2, eye lens structural protein

Gene Symbol

CRYBB2

UniProt

NP_000487.1 (Human)

Additional CRYBB2 Products

Product Documents for CRYBB2 Antibody (1F1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CRYBB2 Antibody (1F1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CRYBB2 Antibody (1F1) - Azide and BSA Free

Customer Reviews for CRYBB2 Antibody (1F1) - Azide and BSA Free (1)

4 out of 5
1 Customer Rating
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4 Stars
100%
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Customer Images

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Mouse eye extract, Wild Type Hek293t, CrybB2 overexpressing Hek293t
    Species: Human
    Verified Customer | Posted 11/25/2014
    Expression of CrybB2 in stably transfected Hek293t cells
    CRYBB2 Antibody (1F1) - Azide and BSA Free H00001415-M02

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Protocols

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FAQs

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