CS Citrate Synthase Antibody (2061) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-43649
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Key Product Details
Species Reactivity
Human, Mouse, Rat, Zebrafish
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 2061
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Citrate synthetase. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CS Citrate Synthase Antibody (2061) - Azide and BSA Free
Western Blot: CS Citrate Synthase Antibody (2061) [NBP2-43649]
Western Blot: CS Citrate Synthase Antibody (2061) [NBP2-43649] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CS Citrate Synthetase Antibody (2061) diluted at 1:1000.Immunocytochemistry/ Immunofluorescence: CS Citrate Synthase Antibody (2061) [NBP2-43649]
Immunocytochemistry/Immunofluorescence: CS Citrate Synthase Antibody (2061) [NBP2-43649] - CS Citrate Synthetase Antibody (2061) detects CS Citrate Synthetase protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Citrate synthetase protein stained by CS Citrate Synthetase Antibody (2061) diluted at 1:500. Blue: Hoechst 33342 staining.Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (2061) [NBP2-43649]
Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (2061) [NBP2-43649] - Analysis of hepatoma. Citrate synthetase antibody [2061] dilution: 1:200.Western Blot: CS Citrate Synthase Antibody (2061) [NBP2-43649]
Western Blot: CS Citrate Synthase Antibody (2061) [NBP2-43649] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE Citrate synthetase antibody dilution: 1:1000Western Blot: CS Citrate Synthase Antibody (2061) [NBP2-43649]
Western Blot: CS Citrate Synthase Antibody (2061) [NBP2-43649] - Analysis of A. 30 ug zebrafish brain lysate/extract 10 % SDS-PAGE Citrate synthetase antibody [2061] dilution: 1:1000Western Blot: CS Citrate Synthase Antibody (2061) [NBP2-43649]
Western Blot: CS Citrate Synthase Antibody (2061) [NBP2-43649] - Analysis of A. 50 ug mouse brain lysate/extract 12 % SDS-PAGE Citrate synthetase antibody [2061] dilution: 1:1000Western Blot: CS Citrate Synthase Antibody (2061) [NBP2-43649]
Western Blot: CS Citrate Synthase Antibody (2061) [NBP2-43649] - CS Citrate Synthetase Antibody (2061) detects CS protein by Western blot analysis.A. 30 ug zebrafish heart lysate/extract. 10 % SDS-PAGE. CS Citrate Synthetase Antibody (2061) dilution: 1:1000.Western Blot: CS Citrate Synthase Antibody (2061) [NBP2-43649] -
Whole Japanese medaka extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthase antibody [GT2061] (NBP2-43649) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Applications for CS Citrate Synthase Antibody (2061) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
10 - 1:500
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: CS Citrate Synthase
Long Name
CS Citrate Synthase
Alternate Names
citrate (Si)-synthase, citrate synthase, mitochondrial, EC 2.3.3.1
Gene Symbol
CS
Additional CS Citrate Synthase Products
Product Documents for CS Citrate Synthase Antibody (2061) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CS Citrate Synthase Antibody (2061) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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