CS Citrate Synthase Antibody (CL2553) - BSA Free

Novus Biologicals | Catalog # NBP2-36773

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2553

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from O75390, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CS Citrate Synthase Antibody (CL2553) - BSA Free

Western Blot: CS Citrate Synthase Antibody (CL2553) [NBP2-36773]

Western Blot: CS Citrate Synthase Antibody (CL2553) [NBP2-36773]

Western Blot: CS Citrate Synthase Antibody (CL2553) [NBP2-36773] - Lane 1: Marker [kDa]. Lane 2:Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: CS Citrate Synthase Antibody (CL2553) [NBP2-36773]

Immunocytochemistry/ Immunofluorescence: CS Citrate Synthase Antibody (CL2553) [NBP2-36773]

Immunocytochemistry/Immunofluorescence: CS Citrate Synthase Antibody (CL2553) [NBP2-36773] - Staining in MCF7 cell line with Anti-CS monoclonal antibody, showing specific mitochondrial staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunohistochemistry: CS Citrate Synthase Antibody (CL2553) [NBP2-36773]

Immunohistochemistry: CS Citrate Synthase Antibody (CL2553) [NBP2-36773]

Immunohistochemistry: CS Citrate Synthase Antibody (CL2553) [NBP2-36773] - Staining of human kidney shows granular cytoplasmic immunoreactivity in renal tubules.
Immunocytochemistry/ Immunofluorescence: CS Citrate Synthase Antibody (CL2553) [NBP2-36773]

Immunocytochemistry/ Immunofluorescence: CS Citrate Synthase Antibody (CL2553) [NBP2-36773]

Immunocytochemistry/Immunofluorescence: CS Citrate Synthase Antibody (CL2553) [NBP2-36773] - Staining in HeLa cell line showing specific mitochondrial staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: CS Citrate Synthase Antibody (CL2553) [NBP2-36773]

Immunocytochemistry/ Immunofluorescence: CS Citrate Synthase Antibody (CL2553) [NBP2-36773]

Immunocytochemistry/Immunofluorescence: CS Citrate Synthase Antibody (CL2553) [NBP2-36773] - Staining of human cell line A431 showing specific mitochondrial staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: CS Citrate Synthase Antibody (CL2553) [NBP2-36773]

Immunocytochemistry/ Immunofluorescence: CS Citrate Synthase Antibody (CL2553) [NBP2-36773]

Immunocytochemistry/Immunofluorescence: CS Citrate Synthase Antibody (CL2553) [NBP2-36773] - Staining in U2OS cell line with Anti-CS monoclonal antibody, showing specific mitochondrial staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: CS Citrate Synthase Antibody (CL2553) [NBP2-36773]

Immunocytochemistry/ Immunofluorescence: CS Citrate Synthase Antibody (CL2553) [NBP2-36773]

Immunocytochemistry/Immunofluorescence: CS Citrate Synthase Antibody (CL2553) [NBP2-36773] - Staining in U251 cell line showing specific mitochondrial staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunohistochemistry: CS Citrate Synthase Antibody (CL2553) [NBP2-36773]

Immunohistochemistry: CS Citrate Synthase Antibody (CL2553) [NBP2-36773]

Immunohistochemistry: CS Citrate Synthase Antibody (CL2553) [NBP2-36773] - Staining of human fallopian tube shows strong granular cytoplasmic immunoreactivity in glandular cells.
Immunohistochemistry: CS Citrate Synthase Antibody (CL2553) [NBP2-36773]

Immunohistochemistry: CS Citrate Synthase Antibody (CL2553) [NBP2-36773]

Immunohistochemistry: CS Citrate Synthase Antibody (CL2553) [NBP2-36773] - Staining of human breast cancer shows strong granular cytoplasmic positivity in tumor cells.
Immunohistochemistry: CS Citrate Synthase Antibody (CL2553) [NBP2-36773]

Immunohistochemistry: CS Citrate Synthase Antibody (CL2553) [NBP2-36773]

Immunohistochemistry: CS Citrate Synthase Antibody (CL2553) [NBP2-36773] - Staining of human rectum shows strong granular cytoplasmic immunoreactivity in glandular cells.
Immunohistochemistry: CS Citrate Synthase Antibody (CL2553) [NBP2-36773]

Immunohistochemistry: CS Citrate Synthase Antibody (CL2553) [NBP2-36773]

Immunohistochemistry: CS Citrate Synthase Antibody (CL2553) [NBP2-36773] - Staining of human small intestine shows granular cytoplasmic immunoreactivity in both glandular and lamina propria cells.
Immunohistochemistry: CS Citrate Synthase Antibody (CL2553) [NBP2-36773]

Immunohistochemistry: CS Citrate Synthase Antibody (CL2553) [NBP2-36773]

Immunohistochemistry: CS Citrate Synthase Antibody (CL2553) [NBP2-36773] - Staining of human prostate shows granular cytoplasmic positivity in glandular cells.

Applications for CS Citrate Synthase Antibody (CL2553) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CS Citrate Synthase

The protein encoded by the CS Citrate Synthase gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citratefrom oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. Thisprotein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. (provided byRefSeq)

Long Name

CS Citrate Synthase

Alternate Names

citrate (Si)-synthase, citrate synthase, mitochondrial, EC 2.3.3.1

Gene Symbol

CS

UniProt

Additional CS Citrate Synthase Products

Product Documents for CS Citrate Synthase Antibody (CL2553) - BSA Free

Certificate of Analysis

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Product Specific Notices for CS Citrate Synthase Antibody (CL2553) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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