CS Citrate Synthase Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-13878

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: ADLIPKEQARIKTFRQQHGKTVVGQITVDMMYGGMRGMKGLVYETSVLDPDEGIRFRGFSIPECQKLLPKAKGGEEPLPEGLFWLLVTGHIPTEEQVSWL

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29290465).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CS Citrate Synthase Antibody - BSA Free

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878]

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878]

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878] - Staining of human cerebral cortex, heart muscle, kidney and pancreas using Anti-CS antibody NBP2-13878 (A) shows similar protein distribution across tissues to independent antibody NBP2-13877 (B).
Western Blot: CS Citrate Synthase Antibody [NBP2-13878]

Western Blot: CS Citrate Synthase Antibody [NBP2-13878]

Western Blot: CS Citrate Synthase Antibody [NBP2-13878] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: CS Citrate Synthase Antibody [NBP2-13878]

Immunocytochemistry/ Immunofluorescence: CS Citrate Synthase Antibody [NBP2-13878]

Immunocytochemistry/Immunofluorescence: CS Citrate Synthase Antibody [NBP2-13878] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878]

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878]

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878] - Staining of human cererbellum shows moderate to strong cytoplasmic positivity in purkinje cells.
Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878]

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878]

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878] - Staining of human heart muscle shows high expression.
Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878]

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878]

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878] - Staining of human kidney.
Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878]

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878]

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878] - Staining of human skeletal muscle shows weak to moderate cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878]

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878]

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878] - Staining of human cerebral cortex.
Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878]

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878]

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878]

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878]

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878] - Staining of human pancreas.

Applications for CS Citrate Synthase Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CS Citrate Synthase

The protein encoded by the CS Citrate Synthase gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citratefrom oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. Thisprotein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. (provided byRefSeq)

Long Name

CS Citrate Synthase

Alternate Names

citrate (Si)-synthase, citrate synthase, mitochondrial, EC 2.3.3.1

Gene Symbol

CS

Additional CS Citrate Synthase Products

Product Documents for CS Citrate Synthase Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CS Citrate Synthase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CS Citrate Synthase Antibody - BSA Free

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Protocols

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