Cultrex In Vitro Angiogenesis Assay (EC Invasion), 96-well

Endothelial cell invasion assay for evaluation of angiogensis
Catalog # Availability Size / Price Qty
3471-096-K
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Cultrex In Vitro Angiogenesis Assay (EC Invasion), 96-well Summary

Allows for the detection of inducers and inhibitors of endothelial cell tube formation.

Why Use Cultrex In Vitro Angiogenesis Assay (EC Invasion), 96-well?

The Cultrex In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit was created in an effort to accelerate the screening process for compounds that influence vascular endothelial cell digestion of and invasion through a basement membrane extract (BME) layer. This kit offers a flexible, standardized, 96 well high-throughput format for quantifying endothelial invasion, in vitro, in response to chemoattractants and/or inhibiting compounds. This assay employs a simplified Boyden chamber-like design with an 8 micron polyethylene terephthalate (PET) membrane. Sulforaphane [1-isothiocyanato-(4R)-methylsulfinyl)-butane], a naturally occurring cancer chemopreventive agent, is provided as a control for inhibition of in vitro endothelial cell invasion through Cultrex BME. Detection of cell invasion is quantified using Calcein-AM, which is internalized by the cells, and converted to the acetomethylester (AM) moiety by intracellular esterases. Free Calcein fluoresces brightly, and this fluorescence may be used to quantify the number of cells that have invaded using a fluorescence plate reader.

Kit Contents

• 5X BME Coating Solution
• Sulforaphane
• Calcein AM
• Cell Invasion Chamber
• 10X Coating Buffer
• 25X Cell Wash Buffer
• 10X Cell Dissociation Solution

Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.

Kit Contents

  • 5X BME Coating Solution
  • Sulforaphane
  • Calcein AM
  • Cell Invasion Chamber
  • 10X Coating Buffer
  • 25X Cell Wash Buffer
  • 10X Cell Dissociation Solution

    Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.

Specifications

Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Reagents Provided

Other Reagents Provided

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