Cultrex Stem Cell Qualified RGF Basement Membrane Extract
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Cultrex Stem Cell Qualified RGF Basement Membrane Extract Summary
- Promotes the attachment of human iPSCs.
- Effectively maintains human iPSCs in a pluripotent state in a feeder-free culture.
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||12 - 18 mg/ml.|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Product is stable for a minimum of 3 months from date of shipment when stored at -20 °C in a manual defrost freezer. For optimal stability, store at -80 °C. Avoid freeze-thaw cycles.
Storage Buffer: Dulbecco’s Modified Eagle’s medium without phenol red, with 10 ug/ml gentamicin sulfate.
Sterility Testing: PathClear® - Negative by PCR test for mycoplasma; 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses. No bacterial or fungal growth detected after incubation at 37 °C for 14 days following USP sterility testing guidelines.
IPSCs were grown for a minimum of 2 passages on eitherCultrex Stem Cell Qualified RGF BME (R&D Systems, Catalog #3434-005-02) or aleading competitor's matrix, prior to creating embryoid bodies forhematopoietic stem cell differentiation. Cells pre-cultured on Cultrex Stem Cell Qualified RGF BME,showed a greater efficiency to develop into hematopoietic stem cells (CD34+,CD45+). Data courtesy of theVerneris Laboratory at the University of Colorado.
Erythroblasts,reprogrammed into induced pluripotent stem cells (iPSCs), were cultured on plates coated with Cultrex Stem CellQualified RGF BME (Catalog # 3434-005-02). Example colonies are shown at 4Xmagnification. Data courtesy of the Harvard Stem Cell Core.
Background: Basement Membrane Extracts
Cultrex® Stem Cell Qualified Basement Membrane Extract (BME) has been shown to provide an effective feeder-free surface for the attachment and maintenance of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) in an undifferentiated state, thereby enabling its use for growth promotion or study of stem cell differentiation.
Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma. Basement membranes are degraded and regenerated during development and wound healing. They not only support cells and cell layers, but they also play an essential role in tissue organization that affects cell adhesion, migration, proliferation, and differentiation. Basement membranes provide major barriers to invasion by metastatic tumor cells.
Cultrex® BME is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycan.
Citation for Cultrex Stem Cell Qualified RGF Basement Membrane Extract
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Use of a synthetic xeno-free culture substrate for induced pluripotent stem cell induction and retinal differentiation.
Authors: Tucker B, Anfinson K, Mullins R, Stone E, Young M
Stem Cells Transl Med, 2013;2(1):16-24.
How does Cultrex® BME promote cell differentiation?
All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic substrates, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.
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